MAF1/YDR005C Summary Help

Standard Name MAF1 1
Systematic Name YDR005C
Feature Type ORF, Verified
Description Highly conserved negative regulator of RNA polymerase III; involved in tRNA processing and stability; inhibits tRNA degradation via rapid tRNA decay (RTD) pathway; binds N-terminal domain of Rpc160p subunit of Pol III to prevent closed-complex formation; localization and activity are regulated by phosphorylation, mediated by TORC1, protein kinase A, and Sch9p; localizes to cytoplasm during vegetative growth and translocates to nucleus and nucleolus under stress conditions (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
Chromosomal Location
ChrIV:458103 to 456836 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All MAF1 GO evidence and references
  View Computational GO annotations for MAF1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Classical genetics
Large-scale survey
171 total interaction(s) for 112 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 15
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 17
  • Biochemical Activity: 10
  • Reconstituted Complex: 2
  • Two-hybrid: 2

Genetic Interactions
  • Dosage Rescue: 1
  • Negative Genetic: 57
  • Phenotypic Enhancement: 1
  • Phenotypic Suppression: 2
  • Positive Genetic: 5
  • Synthetic Growth Defect: 43
  • Synthetic Lethality: 5
  • Synthetic Rescue: 10

Expression Summary
Length (a.a.) 395
Molecular Weight (Da) 44,732
Isoelectric Point (pI) 4.99
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrIV:458103 to 456836 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..6 458103..458098 2011-02-03 1996-07-31
Intron 7..86 458097..458018 2011-02-03 1996-07-31
CDS 87..1268 458017..456836 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002412
References cited on this page View Complete Literature Guide for MAF1
1) Murawski M, et al.  (1994) maf1 mutation alters the subcellular localization of the Mod5 protein in yeast. Acta Biochim Pol 41(4):441-8
2) Boguta M, et al.  (1997) Mutation in a new gene MAF1 affects tRNA suppressor efficiency in Saccharomyces cerevisiae. Gene 185(2):291-6
3) Pluta K, et al.  (2001) Maf1p, a negative effector of RNA polymerase III in Saccharomyces cerevisiae. Mol Cell Biol 21(15):5031-40
4) Upadhya R, et al.  (2002) Maf1 is an essential mediator of diverse signals that repress RNA polymerase III transcription. Mol Cell 10(6):1489-94
5) Desai N, et al.  (2005) Two steps in Maf1-dependent repression of transcription by RNA polymerase III. J Biol Chem 280(8):6455-62
6) Oficjalska-Pham D, et al.  (2006) General repression of RNA polymerase III transcription is triggered by protein phosphatase type 2A-mediated dephosphorylation of Maf1. Mol Cell 22(5):623-32
7) Towpik J, et al.  (2008) Derepression of RNA Polymerase III Transcription by Phosphorylation and Nuclear Export of Its Negative Regulator, Maf1. J Biol Chem 283(25):17168-74
8) Lee J, et al.  (2009) Regulation of RNA Polymerase III Transcription Involves SCH9-dependent and SCH9-independent Branches of the Target of Rapamycin (TOR) Pathway. J Biol Chem 284(19):12604-8
9) Wei Y, et al.  (2009) Mechanisms of regulation of RNA polymerase III-dependent transcription by TORC1. EMBO J 28(15):2220-30
10) Vannini A, et al.  (2010) Molecular basis of RNA polymerase III transcription repression by Maf1. Cell 143(1):59-70
11) Turowski TW, et al.  (2012) Maf1-mediated repression of RNA polymerase III transcription inhibits tRNA degradation via RTD pathway. RNA 18(10):1823-32
12) Wichtowska D, et al.  (2013) An interplay between transcription, processing, and degradation determines tRNA levels in yeast. Wiley Interdiscip Rev RNA 4(6):709-22