Standard Name	UFD2 1
Systematic Name	YDL190C
Feature Type	ORF, Verified
Description	Ubiquitin chain assembly factor (E4) that cooperates with a ubiquitin-activating enzyme (E1), a ubiquitin-conjugating enzyme (E2), and a ubiquitin protein ligase (E3) to conjugate ubiquitin to substrates; also functions as an E3 (2, 3)
Name Description	Ubiquitin Fusion Degradation 1

Chromosomal Location	ChrIV:121592 to 118707 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.

	View Computational GO annotations for UFD2
Molecular Function
Manually curated	ubiquitin-ubiquitin ligase activity (IDA)
Biological Process
Manually curated	ER-associated protein catabolic process (IMP) protein ubiquitination (IDA) response to stress (IMP) ubiquitin-dependent protein catabolic process (IMP, IPI)
Cellular Component
High-throughput	cytoplasm (IDA) nucleus (IDA)

Classical genetics
null	toxin resistance: decreased
Large-scale survey
null	acid pH resistance: decreased auxotrophy competitive fitness: decreased haploproficient heat sensitivity: increased resistance to camptothecin: increased resistance to tacrolimus (anhydrous) and tunicamycin: increased resistance to hydroxyurea: increased resistance to 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid: decreased resistance to fluconazole: decreased resistance to methyl methanesulfonate: decreased resistance to sodium selenide: decreased resistance to 1,4-dithiothreitol and tacrolimus (anhydrous): increased resistance to doxorubicin: decreased sporulation: decreased sporulation efficiency: decreased vacuolar morphology: abnormal vegetative growth: decreased rate viable
overexpression	vegetative growth: decreased rate
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	173 total interaction(s) for 91 unique genes/features.
Physical Interactions	Affinity Capture-MS: 22 Affinity Capture-Western: 25 Biochemical Activity: 1 Co-purification: 6 Protein-peptide: 1 Reconstituted Complex: 8 Two-hybrid: 16
Genetic Interactions	Dosage Growth Defect: 1 Dosage Lethality: 1 Negative Genetic: 46 Phenotypic Enhancement: 9 Phenotypic Suppression: 14 Positive Genetic: 9 Synthetic Growth Defect: 6 Synthetic Lethality: 3 Synthetic Rescue: 5
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | CGD Homologs | CandidaDB Homologs | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrIV:121592 to 118707 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.
Last Update	Coordinates: 2011-02-03 | Sequence: 2011-02-03
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..2886 121592..118707 2011-02-03 2011-02-03
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CBS7960 S.c. Strain CEN.PK113-7D S.c. Strain CLIB215 S.c. Strain CLIB324 S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain T73 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000002349