GLT1/YDL171C Summary Help

Standard Name GLT1 1
Systematic Name YDL171C
Feature Type ORF, Verified
Description NAD(+)-dependent glutamate synthase (GOGAT); synthesizes glutamate from glutamine and alpha-ketoglutarate; with Gln1p, forms the secondary pathway for glutamate biosynthesis from ammonia; expression regulated by nitrogen source; assembles into filaments as cells approach stationary phase and under cytosolic acidification and starvation conditions (2, 3, 4 and see Summary Paragraph)
Name Description GLuTamate synthase
Chromosomal Location
ChrIV:155640 to 149203 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All GLT1 GO evidence and references
  View Computational GO annotations for GLT1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Regulators 8 genes
Classical genetics
Large-scale survey
60 total interaction(s) for 56 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 30
  • Affinity Capture-RNA: 2
  • Biochemical Activity: 1
  • Co-localization: 1

Genetic Interactions
  • Dosage Rescue: 1
  • Negative Genetic: 18
  • Phenotypic Enhancement: 1
  • Positive Genetic: 2
  • Synthetic Growth Defect: 1
  • Synthetic Lethality: 2
  • Synthetic Rescue: 1

Expression Summary
Length (a.a.) 2,145
Molecular Weight (Da) 238,100
Isoelectric Point (pI) 6.55
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrIV:155640 to 149203 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..6438 155640..149203 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002330

About glutamate biosynthesis from glutamine

Yeast cells contain 3 pathways for the synthesis of glutamate. Two pathways are mediated by two isoforms of glutamate dehydrogenase, encoded by GDH1 and GDH3 (5, 6). The third pathway is driven by the combined activities of glutamine synthetase and glutamate synthase, encoded by GLN1 and GLT1, respectively (7, 1). Gln1p catalyzes amination of glutamate to form glutamine; Glt1p then transfers the amide group of glutamine to 2-oxoglutarate, generating two molecules of glutamate. Glutamate synthase, also referred to as GOGAT, is a trimer of three Glt1p subunits. Expression of the GLT1 gene is modulated by glutamate-mediated repression and by Gln3p/Gcn4p-mediated activation, depending upon the availability of nitrogen and glutamate in the medium. In amino acid starvation conditions, GLT1 expression is activated to a moderate degree by Gcn4p (2).

Last updated: 2007-10-04 Contact SGD

References cited on this page View Complete Literature Guide for GLT1
1) Filetici P, et al.  (1996) Sequence of the GLT1 gene from Saccharomyces cerevisiae reveals the domain structure of yeast glutamate synthase. Yeast 12(13):1359-66
2) Valenzuela L, et al.  (1998) Regulation of expression of GLT1, the gene encoding glutamate synthase in Saccharomyces cerevisiae. J Bacteriol 180(14):3533-40
3) Magasanik B  (1992) "Regulation of Nitrogen Utilization." Pp. 283-317 in the Molecular and Cellular Biology of the Yeast Saccharomyces: Gene Expression, edited by Jones EW, Pringle JR and Broach JR. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press
4) Petrovska I, et al.  (2014) Filament formation by metabolic enzymes is a specific adaptation to an advanced state of cellular starvation. Elife ()
5) Moye WS, et al.  (1985) Nucleotide sequence of yeast GDH1 encoding nicotinamide adenine dinucleotide phosphate-dependent glutamate dehydrogenase. J Biol Chem 260(14):8502-8
6) Avendano A, et al.  (1997) GDH3 encodes a glutamate dehydrogenase isozyme, a previously unrecognized route for glutamate biosynthesis in Saccharomyces cerevisiae. J Bacteriol 179(17):5594-7
7) Benjamin PM, et al.  (1989) Three regulatory systems control expression of glutamine synthetase in Saccharomyces cerevisiae at the level of transcription. Mol Gen Genet 217(2-3):370-7