ENT1/YDL161W Summary Help

Standard Name ENT1 1
Systematic Name YDL161W
Feature Type ORF, Verified
Description Epsin-like protein involved in endocytosis and actin patch assembly; functionally redundant with Ent2p; binds clathrin via a clathrin-binding domain motif at C-terminus; relocalizes from bud neck to cytoplasm upon DNA replication stress; ENT1 has a paralog, ENT2, that arose from the whole genome duplication (1, 2, 3)
Name Description Epsin N-Terminal homology 1
Chromosomal Location
ChrIV:167714 to 169078 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All ENT1 GO evidence and references
  View Computational GO annotations for ENT1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 4 genes
Resources
Classical genetics
null
Large-scale survey
null
overexpression
Resources
52 total interaction(s) for 27 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 5
  • Affinity Capture-Western: 8
  • Co-localization: 1
  • Reconstituted Complex: 4
  • Two-hybrid: 10

Genetic Interactions
  • Dosage Rescue: 3
  • Negative Genetic: 9
  • Phenotypic Enhancement: 3
  • Phenotypic Suppression: 2
  • Positive Genetic: 1
  • Synthetic Growth Defect: 3
  • Synthetic Lethality: 3

Resources
Expression Summary
histogram
Resources
Length (a.a.) 454
Molecular Weight (Da) 52,352
Isoelectric Point (pI) 5.66
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIV:167714 to 169078 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1365 167714..169078 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002320
References cited on this page View Complete Literature Guide for ENT1
1) Wendland B, et al.  (1999) Yeast epsins contain an essential N-terminal ENTH domain, bind clathrin and are required for endocytosis. EMBO J 18(16):4383-93
2) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
3) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76