DHH1/YDL160C Summary Help

DHH1 BASIC INFORMATION

Standard Name DHH1
Systematic Name YDL160C
Feature Type ORF, Verified
Description Cytoplasmic DExD/H-box helicase, stimulates mRNA decapping, coordinates distinct steps in mRNA function and decay, interacts with both the decapping and deadenylase complexes, may have a role in mRNA export and translation (1, 2, 3 and see Summary Paragraph)
Name Description DExD/H-box Helicase
GO Annotations All DHH1 GO evidence and references
    View Computational GO annotations for DHH1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Mutant Phenotype All DHH1 Phenotype details and references
Classical genetics
null
Large-scale survey
null
overexpression
Interactions DHH1 All interactions details and references
137 total interaction(s) for 78 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 44
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 7
  • Co-purification: 1
  • PCA: 24
  • Protein-RNA: 1
  • Reconstituted Complex: 3
  • Two-hybrid: 10
Genetic Interactions
  • Dosage Lethality: 7
  • Dosage Rescue: 11
  • Phenotypic Suppression: 2
  • Synthetic Growth Defect: 10
  • Synthetic Lethality: 10
  • Synthetic Rescue: 6
Sequence Information
ChrIV:171931 to 170411 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Last Update Coordinates: 1996-07-31 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..1521 171931..170411 1996-07-31 1996-07-31
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | UniProtKB
Primary SGDIDS000002319

DHH1 RESOURCES

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SGD ORF mapGBrowse
SGD ORF map
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  • Localization Resources
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  • Functional Analysis

Click on histogram for expression summary
Expression Summary histogram

ADDITIONAL INFORMATION for DHH1

SUMMARY PARAGRAPH for DHH1

DHH1 encodes a highly conserved DEAD-box RNA helicase that associates with components of mRNA decapping, deadenylation, and transcription complexes (1, 4, 5). Dhh1p stimulates mRNA decapping by decapping enzyme Dcp1p, and has been shown to localize to discrete cytoplasmic foci known as P-bodies, which appear to be involved in sequestering mRNAs in a nontranslating pool, from which they are subsequently degraded or possibly reactivated for translation (6). Orthologs of Dhh1p in other eukaryotes, such as Xenopus and Drosophila, play roles in repressing translation of specific mRNAs during early development (7, 8).

DHH1 in yeast is important in release from DNA-damage-induced cell cycle arrest at the G1/S checkpoint (3). dhh1 mutants are not defective for DNA repair and recover normally from the G2/M and replication checkpoints, suggesting a specific function for Dhh1p in recovery from G1/S checkpoint arrest (3). Dhh1p has been suggested to play a role in partitioning mRNAs between translatable and nontranslatable pools, which has been implicated in the recovery from G1/S cell cycle arrest following DNA damage (3). DHH1 is orthologous to the human putative proto-oncogene p54/RCK, and the high degree of conservation between these orthologs suggests that this mechanism is conserved among all eukaryotes and potentially important in human disease (3).

Last updated: 2005-08-06

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for DHH1]

1) Coller JM, et al.  (2001) The DEAD box helicase, Dhh1p, functions in mRNA decapping and interacts with both the decapping and deadenylase complexes. RNA 7(12):1717-27
2) Tseng-Rogenski SS, et al.  (2003) Functional conservation of Dhh1p, a cytoplasmic DExD/H-box protein present in large complexes. Nucleic Acids Res 31(17):4995-5002
3) Bergkessel M and Reese JC  (2004) An essential role for the Saccharomyces cerevisiae DEAD-box helicase DHH1 in G1/S DNA-damage checkpoint recovery. Genetics 167(1):21-33
4) Fischer N and Weis K  (2002) The DEAD box protein Dhh1 stimulates the decapping enzyme Dcp1. EMBO J 21(11):2788-97
5) Maillet L and Collart MA  (2002) Interaction between Not1p, a component of the Ccr4-not complex, a global regulator of transcription, and Dhh1p, a putative RNA helicase. J Biol Chem 277(4):2835-42
6) Sheth U and Parker R  (2003) Decapping and decay of messenger RNA occur in cytoplasmic processing bodies. Science 300(5620):805-8
7) Smillie DA and Sommerville J  (2002) RNA helicase p54 (DDX6) is a shuttling protein involved in nuclear assembly of stored mRNP particles. J Cell Sci 115(Pt 2):395-407
8) Nakamura A, et al.  (2001) Me31B silences translation of oocyte-localizing RNAs through the formation of cytoplasmic RNP complex during Drosophila oogenesis. Development 128(17):3233-42