STE7/YDL159W Summary Help

Standard Name STE7
Systematic Name YDL159W
Feature Type ORF, Verified
Description Signal transducing MAP kinase kinase involved in pheromone response, where it phosphorylates Fus3p, and in the pseudohyphal/invasive growth pathway, through phosphorylation of Kss1p; phosphorylated by Ste11p, degraded by ubiquitin pathway (1, 2 and see Summary Paragraph)
Name Description STErile
Chromosomal Location
ChrIV:172481 to 174028 | ORF Map | GBrowse
Gbrowse
Genetic position: -76 cM
Gene Ontology Annotations All STE7 GO evidence and references
  View Computational GO annotations for STE7
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Mutant phenotypes All STE7 Phenotype evidence and references
Classical genetics
conditional
null
unspecified
Large-scale survey
null
Resources
interactions All STE7 Interaction evidence and references
112 total interaction(s) for 43 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 14
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 14
  • Biochemical Activity: 8
  • Far Western: 1
  • FRET: 8
  • PCA: 7
  • Protein-peptide: 1
  • Reconstituted Complex: 16
  • Two-hybrid: 16
Genetic Interactions
  • Dosage Rescue: 2
  • Phenotypic Enhancement: 7
  • Phenotypic Suppression: 8
  • Synthetic Growth Defect: 1
  • Synthetic Lethality: 1
  • Synthetic Rescue: 5
Resources
Expression Summary
histogram
Resources
Protein Information All STE7 Protein evidence and references
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIV:172481 to 174028 | ORF Map | GBrowse
This feature contains embedded feature(s): YDL159C-B
SGD ORF map
Genetic position: -76 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..1548 172481..174028 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002318
SUMMARY PARAGRAPH for STE7

Ste7p is a member of the MAP kinase (MAPK or MEK) family that is involved in both the mating and filamentous growth signaling pathways (3, 4). Eukaryotic cells use mitogen-activated protein kinase (MAPK) cascades to sense changes in their environment and transmit the signal to the nucleus to activate the proper response. Yeast cells use a conserved set of three MAP kinases to induce both the mating and filamentous growth pathways. Two of the kinases, Ste11p (MAPKK or MEKK) and Ste7p (MAPKK or MEK), are used in both pathways. Fus3p (MAPK) functions primarily in mating while Kss1p is involved in filamentation (5). The Ste5 protein serves as a scaffold that binds Ste11p, Ste7p, and Fus3p to ensure pathway specificity during the mating response (6). Both genetic and biochemical experiments have shown that the Ste11 kinase phosphorylates and activates Ste7p (7, 8), then Ste7p subsequently phosphorylates Fus3p at threonine-180 and tyrosine-182 (9, 10). The kinase cascade culminates with the activation of the transcription factor Ste12p by Fus3p. (11, 12).

Last updated: 1999-10-21

References cited on this page View Complete Literature Guide for STE7
1) O'Rourke SM and Herskowitz I  (1998) The Hog1 MAPK prevents cross talk between the HOG and pheromone response MAPK pathways in Saccharomyces cerevisiae. Genes Dev 12(18):2874-86
2) Wang Y, et al.  (2003) Regulation of Ste7 ubiquitination by Ste11 phosphorylation and the Skp1-Cullin-F-box complex. J Biol Chem 278(25):22284-9
3) Teague MA, et al.  (1986) Nucleotide sequence of the yeast regulatory gene STE7 predicts a protein homologous to protein kinases. Proc Natl Acad Sci U S A 83(19):7371-5
4) Mosch HU, et al.  (1996) Ras2 signals via the Cdc42/Ste20/mitogen-activated protein kinase module to induce filamentous growth in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A 93(11):5352-6
5) Schaeffer HJ and Weber MJ  (1999) Mitogen-activated protein kinases: specific messages from ubiquitous messengers. Mol Cell Biol 19(4):2435-44
6) Printen JA and Sprague GF Jr  (1994) Protein-protein interactions in the yeast pheromone response pathway: Ste5p interacts with all members of the MAP kinase cascade. Genetics 138(3):609-19
7) Neiman AM and Herskowitz I  (1994) Reconstitution of a yeast protein kinase cascade in vitro: activation of the yeast MEK homologue STE7 by STE11. Proc Natl Acad Sci U S A 91(8):3398-402
8) Zheng CF and Guan KL  (1994) Activation of MEK family kinases requires phosphorylation of two conserved Ser/Thr residues. EMBO J 13(5):1123-31
9) Errede B, et al.  (1993) MAP kinase-related FUS3 from S. cerevisiae is activated by STE7 in vitro. Nature 362(6417):261-4
10) Gartner A, et al.  (1992) Signal transduction in Saccharomyces cerevisiae requires tyrosine and threonine phosphorylation of FUS3 and KSS1. Genes Dev 6(7):1280-92
11) Elion EA, et al.  (1993) FUS3 phosphorylates multiple components of the mating signal transduction cascade: evidence for STE12 and FAR1. Mol Biol Cell 4(5):495-510
12) Elion EA, et al.  (1991) FUS3 represses CLN1 and CLN2 and in concert with KSS1 promotes signal transduction. Proc Natl Acad Sci U S A 88(21):9392-6