SAS10/YDL153C Summary Help

Standard Name SAS10 1
Systematic Name YDL153C
Alias UTP3 2
Feature Type ORF, Verified
Description Subunit of U3-containing Small Subunit (SSU) processome complex; involved in production of 18S rRNA and assembly of small ribosomal subunit; disrupts silencing when overproduced; mutant has increased aneuploidy tolerance; essential gene (1, 2, 3 and see Summary Paragraph)
Name Description Something About Silencing 1
Chromosomal Location
ChrIV:183018 to 181186 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All SAS10 GO evidence and references
  View Computational GO annotations for SAS10
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 5 genes
Resources
Classical genetics
null
overexpression
repressible
Large-scale survey
null
overexpression
reduction of function
Resources
70 total interaction(s) for 62 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 19
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 1
  • Biochemical Activity: 26
  • Reconstituted Complex: 1
  • Two-hybrid: 17

Genetic Interactions
  • Negative Genetic: 2
  • Synthetic Growth Defect: 2
  • Synthetic Lethality: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 610
Molecular Weight (Da) 70,259
Isoelectric Point (pI) 4.42
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIV:183018 to 181186 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1833 183018..181186 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002312
SUMMARY PARAGRAPH for SAS10

About the early stages of rRNA processing and 40S small ribosomal subunit assembly

The early stages of ribosome assembly occur in conjunction with processing of the 35S pre-ribosomal RNA transcript into the mature 18S, 5.8S, and 25S rRNA molecules. The first three cleavages at A0, A1, and A2 (see diagram) are essential for production of the 18S rRNA and the 40S small ribosomal subunit, but mutations which interfere with these cleavages have little effect on production of the 60S large ribosomal subunit (4). These three early cleavages occur in a series of large U3-associated ribonucleoprotein complexes (5, 6) and require base pairing of the U3 snoRNA with sequences in the 5'-ETS and the 18S rRNA (7, 8).

Click on the following figure for more details about the rDNA repeat and cleavage sites within the rRNA transcript:

figure 1

About the 90S preribosome and SSU processome complexes

A number of U3-containing early ribosome assembly and rRNA processing complexes have been identified that contain the 35S pre-rRNA transcript and have overlapping but not identical protein compositions (5, 6). Both the 90S preribosome and the small subunit (SSU) processome complexes contain ribosomal proteins, primarily of the small subunit, and non-ribosomal proteins presumably involved in rRNA processing and assembly of the small 40S ribsomal subunit. While many proteins are found in both complexes, some are found in only one or the other (see lists below). It may be that the 90S preribosome and SSU processome complexes are both intermediates in a series of complexes leading to the assembly of the small ribosomal subunit (5), or it may be that the SSU processome lies on an alternate assembly pathway (6).

The 90S preribosome complex is described as corresponding to the earliest detectable rRNA processing and ribosome assembly complex (9). The 90S is itself assembled from a number of stable subcomplexes including the t-UTP subcomplex (Utp5p, Utp4p, Nan1p, Utp8p, Utp9p, Utp10p, and Utp15p), the Pwp2p/UTP-B subcomplex (Utp6p, Pwp2p, Utp18p, Utp21p, Utp13p, and Dip2p) which interacts directly with the 5'-ETS of the 35S pre-rRNA (10), the UTP-C subcomplex (Rrp7p, Utp22p, Ckb1p, Cka1p, Ckb2p, and Cka2p), and the Mpp10 subcomplex (Mpp10p, Imp3p, and Imp4p) (11). The t-UTP subcomplex is also found as part of the SSU processome complex, which is slightly smaller at 80S (2, 12). Depletion of any of the members of the t-UTP subcomplex results in decreased transcription of rDNA leading to decreased levels of the primary 35S rRNA transcript (13). In contrast, mutation or depletion of most other members of either the 90S preribosome or SSU processome complexes causes decreased 18S rRNA levels without affecting the levels of the 25S or 5.8S rRNAs.

Non-ribosomal protein components of the 90S preribosome and SSU processome

Subunits of both the 90S preribosome (9) and SSU processome (2, 12) include: Bud21p, Dip2p, Ecm16p, Emg1p, Imp3p, Imp4p, Krr1p, Mpp10p, Nan1p, Noc4p, Nop1p, Nop14p, Nop58p, Pwp2p, Rrp5p, Rrp9p, Nop56p, Sof1p, Utp4p, Utp6p, Utp7p, Utp8p, Utp9p, Utp10p, Utp13p, Utp15p, Utp18p, Utp20p, Utp21p, and Utp22p

Additional subunits of the 90S preribosome (9) include: Bfr2p, Bms1p, Cbf5p, Cms1p, Dbp8p, Dim1p, Enp1p, Enp2p, Has1p, Kre33p, Mrd1p, Nop9p (14), Pno1p, Prp43p, Rcl1p, Rok1p, Rrp12p, Scl1p, Slx9p (15), Tsr1p, and Utp30p

Additional subunits of the SSU processome (2, 12) include: Fcf1p, Utp23p, Sas10p, Snu13p, Utp5p, Utp11p, and Utp14p

Last updated: 2008-07-17 Contact SGD

References cited on this page View Complete Literature Guide for SAS10
1) Kamakaka RT and Rine J  (1998) Sir- and silencer-independent disruption of silencing in Saccharomyces by Sas10p. Genetics 149(2):903-14
2) Dragon F, et al.  (2002) A large nucleolar U3 ribonucleoprotein required for 18S ribosomal RNA biogenesis. Nature 417(6892):967-70
3) Torres EM, et al.  (2010) Identification of aneuploidy-tolerating mutations. Cell 143(1):71-83
4) Venema J and Tollervey D  (1999) Ribosome synthesis in Saccharomyces cerevisiae. Annu Rev Genet 33:261-311
5) Fromont-Racine M, et al.  (2003) Ribosome assembly in eukaryotes. Gene 313:17-42
6) Granneman S and Baserga SJ  (2004) Ribosome biogenesis: of knobs and RNA processing. Exp Cell Res 296(1):43-50
7) Beltrame M and Tollervey D  (1995) Base pairing between U3 and the pre-ribosomal RNA is required for 18S rRNA synthesis. EMBO J 14(17):4350-6
8) Hughes JM  (1996) Functional base-pairing interaction between highly conserved elements of U3 small nucleolar RNA and the small ribosomal subunit RNA. J Mol Biol 259(4):645-54
9) Grandi P, et al.  (2002) 90S pre-ribosomes include the 35S pre-rRNA, the U3 snoRNP, and 40S subunit processing factors but predominantly lack 60S synthesis factors. Mol Cell 10(1):105-15
10) Dosil M and Bustelo XR  (2004) Functional characterization of Pwp2, a WD family protein essential for the assembly of the 90 S pre-ribosomal particle. J Biol Chem 279(36):37385-97
11) Perez-Fernandez J, et al.  (2007) The 90S preribosome is a multimodular structure that is assembled through a hierarchical mechanism. Mol Cell Biol 27(15):5414-29
12) Bernstein KA, et al.  (2004) The small-subunit processome is a ribosome assembly intermediate. Eukaryot Cell 3(6):1619-26
13) Gallagher JE, et al.  (2004) RNA polymerase I transcription and pre-rRNA processing are linked by specific SSU processome components. Genes Dev 18(20):2506-17
14) Thomson E, et al.  (2007) Nop9 is an RNA binding protein present in pre-40S ribosomes and required for 18S rRNA synthesis in yeast. RNA 13(12):2165-2174
15) Bax R, et al.  (2006) Slx9p facilitates efficient ITS1 processing of pre-rRNA in Saccharomyces cerevisiae. RNA 12(11):2005-13