RPN5/YDL147W Summary Help

Standard Name RPN5 1
Systematic Name YDL147W
Alias NAS5
Feature Type ORF, Verified
Description Subunit of the CSN and 26S proteasome lid complexes; similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p; Rpn5p is an essential protein; the COP9 signalosome is also known as the CSN (2, 3, 4)
Name Description Regulatory Particle Non-ATPase 1
Chromosomal Location
ChrIV:190924 to 192261 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All RPN5 GO evidence and references
  View Computational GO annotations for RPN5
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 6 genes
Resources
Classical genetics
conditional
Large-scale survey
conditional
null
reduction of function
unspecified
Resources
347 total interaction(s) for 114 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 249
  • Affinity Capture-RNA: 4
  • Affinity Capture-Western: 19
  • Co-purification: 7
  • PCA: 4
  • Reconstituted Complex: 5
  • Two-hybrid: 14

Genetic Interactions
  • Negative Genetic: 32
  • Positive Genetic: 9
  • Synthetic Growth Defect: 1
  • Synthetic Haploinsufficiency: 1
  • Synthetic Lethality: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 445
Molecular Weight (Da) 51,768
Isoelectric Point (pI) 6.05
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIV:190924 to 192261 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1338 190924..192261 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002306
References cited on this page View Complete Literature Guide for RPN5
1) Finley D, et al.  (1998) Unified nomenclature for subunits of the Saccharomyces cerevisiae proteasome regulatory particle. Trends Biochem Sci 23(7):244-5
2) Saito A, et al.  (1997) cDNA cloning and functional analysis of p44.5 and p55, two regulatory subunits of the 26S proteasome. Gene 203(2):241-50
3) Glickman MH, et al.  (1998) The regulatory particle of the Saccharomyces cerevisiae proteasome. Mol Cell Biol 18(6):3149-62
4) Yu Z, et al.  (2011) Dual function of Rpn5 in two PCI complexes, the 26S proteasome and COP9 signalosome. Mol Biol Cell 22(7):911-20