SCM3/YDL139C Summary

SCM3/YDL139C Summary

Standard Name	SCM3 1
Systematic Name	YDL139C
Feature Type	ORF, Verified
Description	Nonhistone component of centromeric chromatin; binds to histone H3 variant, Cse4p, and recruits it to centromeres; involved in the assembly and maintenance of Cse4-H4 at centromeres; required for kinetochore assembly and G2/M progression; may protect Cse4p from ubiquitylation; homolog of mammalian HJURP (1, 2, 3, 4, 5, 6, 7)
Name Description	Suppressor of Chromosome Missegregation 2

Chromosomal Location	ChrIV:212046 to 211375 \| ORF Map \| GBrowse
	Note: this feature is encoded on the Crick strand.

Gene Ontology Annotations All SCM3 GO evidence and references

	View Computational GO annotations for SCM3
Molecular Function
Manually curated	centromere-specific nucleosome binding (IMP, IPI)
Biological Process
Manually curated	chromosome segregation (IMP) G2/M transition of mitotic cell cycle (IMP) kinetochore assembly (IMP) negative regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process (IDA) protein localization to chromosome, centromeric region (IMP)
Cellular Component
Manually curated	condensed nuclear chromosome, centromeric region (IDA, IPI)
High-throughput	cytoplasm (IDA) nucleus (IDA)

Mutant phenotypes All SCM3 Phenotype evidence and references

Classical genetics
conditional	cell cycle progression through the G2/M phase transition: arrested Cse4p-GFP distribution: abnormal
null	chromosome segregation: abnormal
repressible	cell cycle progression in metaphase: arrested chromosome segregation: abnormal Cse4p-12Myc distribution: abnormal histone H3 distribution: abnormal Mif2p-13Myc distribution: abnormal Cbf2p-13Myc distribution: abnormal spindle morphology: abnormal
Large-scale survey
null	haploinsufficient inviable
overexpression	vegetative growth: decreased
reduction of function	colony sectoring: increased competitive fitness: increased
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All SCM3 Interaction evidence and references

	53 total interaction(s) for 29 unique genes/features.
Physical Interactions	Affinity Capture-MS: 3 Affinity Capture-RNA: 1 Affinity Capture-Western: 9 Biochemical Activity: 1 Co-localization: 1 Reconstituted Complex: 6 Two-hybrid: 15
Genetic Interactions	Dosage Growth Defect: 1 Dosage Rescue: 7 Phenotypic Enhancement: 1 Phenotypic Suppression: 3 Synthetic Growth Defect: 5
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All SCM3 Protein evidence and references

Localization	YeastRC \| Organelle DB (UMich) \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP
Homologs	Ashbya (AGD) \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrIV:212046 to 211375 | |

Note: this feature is encoded on the Crick strand.

SGD ORF map

Last Update

Coordinates: 2011-02-03 | Sequence: 2003-09-22

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..672	212046..211375	2011-02-03	2003-09-22

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000002298

References cited on this page View Complete Literature Guide for SCM3

1)	Chen Y, et al. (2000) The N terminus of the centromere H3-like protein Cse4p performs an essential function distinct from that of the histone fold domain. Mol Cell Biol 20(18):7037-48
2)	Stoler S, et al. (2007) Scm3, an essential Saccharomyces cerevisiae centromere protein required for G2/M progression and Cse4 localization. Proc Natl Acad Sci U S A 104(25):10571-6
3)	Camahort R, et al. (2007) Scm3 is essential to recruit the histone h3 variant cse4 to centromeres and to maintain a functional kinetochore. Mol Cell 26(6):853-65
4)	Mizuguchi G, et al. (2007) Nonhistone Scm3 and histones CenH3-H4 assemble the core of centromere-specific nucleosomes. Cell 129(6):1153-64
5)	Aravind L, et al. (2007) Domain architectures of the Scm3p protein provide insights into centromere function and evolution. Cell Cycle 6(20):2511-5
6)	Hewawasam G, et al. (2010) Psh1 Is an E3 Ubiquitin Ligase that Targets the Centromeric Histone Variant Cse4. Mol Cell 40(3):444-54
7)	Mishra PK, et al. (2011) Misregulation of Scm3p/HJURP Causes Chromosome Instability in Saccharomyces cerevisiae and Human Cells. PLoS Genet 7(9):e1002303