VCX1/YDL128W Summary Help

Standard Name VCX1 1
Systematic Name YDL128W
Alias HUM1 2 , MNR1 3
Feature Type ORF, Verified
Description Vacuolar membrane antiporter with Ca2+/H+ and K+/H+ exchange activity; involved in control of cytosolic Ca2+ and K+ concentrations; has similarity to sodium/calcium exchangers, including the bovine Na+/Ca2+,K+ antiporter (1, 2, 4, 5 and see Summary Paragraph)
Name Description VaCuolar H+/Ca2+ eXchanger 1
Chromosomal Location
ChrIV:232652 to 233887 | ORF Map | GBrowse
Gene Ontology Annotations All VCX1 GO evidence and references
  View Computational GO annotations for VCX1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 6 genes
Classical genetics
gain of function
Large-scale survey
33 total interaction(s) for 26 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 3
  • Affinity Capture-RNA: 1
  • Biochemical Activity: 6
  • PCA: 4

Genetic Interactions
  • Dosage Rescue: 4
  • Negative Genetic: 3
  • Phenotypic Enhancement: 3
  • Synthetic Growth Defect: 4
  • Synthetic Lethality: 4
  • Synthetic Rescue: 1

Expression Summary
Length (a.a.) 411
Molecular Weight (Da) 44,646
Isoelectric Point (pI) 5.54
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrIV:232652 to 233887 | ORF Map | GBrowse
Last Update Coordinates: 1996-07-31 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..1236 232652..233887 1996-07-31 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | TCDB | UniProtKB
Primary SGDIDS000002286

VCX1 encodes a vacuolar H+/Ca2+ exchanger which is involved in calcium homeostasis and signal transduction. Vcx1p is responsible for restoring cytosolic Ca2+ levels in response to a brief Ca2+ burst. S. cerevisiae has three Ca2+ transporters: Vcx1p, a high capacity, low affinity vacuolar Ca2+ exchanger; Pmc1p, a high-affinity vacuolar Ca2+ ATPase; and Pmr1p, a Golgi Ca2+ ATPase (2, 1, 4).

Vcx1p and Pmc1p are involved in vacuolar Ca2+ accumulation and are both regulated by calcineurin although the mechanisms are different. Calcineurin induces expression of PMC1 and PMR1 through the action of the transcription factor Crz1p, whereas Vcx1p activity is negatively regulated by calcineurin in a Crz1p-independent mechanism (6, 7). Inhibition of Vcx1p by calcineurin is thought to be by a post-translational mechanism (1). Regulation of Vcx1p also involves Rcn1p that acts as an endogenous feedback inhibitor of calcineurin (8).

Vcx1p shares sequence similarity with mammalian and plant Ca2+ transporters (2, 9).

Last updated: 2006-11-20 Contact SGD

References cited on this page View Complete Literature Guide for VCX1
1) Cunningham KW and Fink GR  (1996) Calcineurin inhibits VCX1-dependent H+/Ca2+ exchange and induces Ca2+ ATPases in Saccharomyces cerevisiae. Mol Cell Biol 16(5):2226-37
2) Pozos TC, et al.  (1996) The product of HUM1, a novel yeast gene, is required for vacuolar Ca2+/H+ exchange and is related to mammalian Na+/Ca2+ exchangers. Mol Cell Biol 16(7):3730-41
3) del Pozo L, et al.  (1999) A single nucleotide change in the MNR1 (VCX1/HUM1) gene determines resistance to manganese in Saccharomyces cerevisiae. Yeast 15(5):371-5
4) Miseta A, et al.  (1999) The vacuolar Ca2+/H+ exchanger Vcx1p/Hum1p tightly controls cytosolic Ca2+ levels in S. cerevisiae. FEBS Lett 451(2):132-6
5) Cagnac O, et al.  (2010) Vacuolar cation/H+ antiporters of Saccharomyces cerevisiae. J Biol Chem 285(44):33914-22
6) Stathopoulos AM and Cyert MS  (1997) Calcineurin acts through the CRZ1/TCN1-encoded transcription factor to regulate gene expression in yeast. Genes Dev 11(24):3432-44
7) Matheos DP, et al.  (1997) Tcn1p/Crz1p, a calcineurin-dependent transcription factor that differentially regulates gene expression in Saccharomyces cerevisiae. Genes Dev 11(24):3445-58
8) Kingsbury TJ and Cunningham KW  (2000) A conserved family of calcineurin regulators. Genes Dev 14(13):1595-604
9) Pittman JK, et al.  (2002) Distinct N-terminal regulatory domains of Ca(2+)/H(+) antiporters. Plant Physiol 130(2):1054-62