CYK3/YDL117W Summary Help

Standard Name CYK3 1
Systematic Name YDL117W
Feature Type ORF, Verified
Description SH3-domain protein located in the bud neck and cytokinetic actin ring; relocalizes from bud neck to nucleus upon DNA replication stress; activates the chitin synthase activity of Chs2p during cytokinesis; suppressor of growth and cytokinesis defects of chs2 phospho-mutants (1, 2, 3, 4)
Name Description CYtoKinesis 1
Chromosomal Location
ChrIV:248581 to 251238 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All CYK3 GO evidence and references
  View Computational GO annotations for CYK3
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 3 genes
Resources
Classical genetics
null
Large-scale survey
null
overexpression
Resources
264 total interaction(s) for 151 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 4
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 1
  • Biochemical Activity: 1
  • PCA: 9
  • Protein-RNA: 1
  • Reconstituted Complex: 3
  • Two-hybrid: 21

Genetic Interactions
  • Dosage Growth Defect: 6
  • Dosage Rescue: 14
  • Negative Genetic: 128
  • Phenotypic Enhancement: 4
  • Phenotypic Suppression: 2
  • Positive Genetic: 14
  • Synthetic Growth Defect: 30
  • Synthetic Lethality: 21
  • Synthetic Rescue: 4

Resources
Expression Summary
histogram
Resources
Length (a.a.) 885
Molecular Weight (Da) 100,620
Isoelectric Point (pI) 9.64
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIV:248581 to 251238 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2004-02-11 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2658 248581..251238 2004-02-11 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002275
References cited on this page View Complete Literature Guide for CYK3
1) Korinek WS, et al.  (2000) Cyk3, a novel SH3-domain protein, affects cytokinesis in yeast. Curr Biol 10(15):947-50
2) Oh Y, et al.  (2012) Mitotic exit kinase Dbf2 directly phosphorylates chitin synthase Chs2 to regulate cytokinesis in budding yeast. Mol Biol Cell 23(13):2445-56
3) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
4) Devrekanli A, et al.  (2012) Inn1 and Cyk3 regulate chitin synthase during cytokinesis in budding yeasts. J Cell Sci 125(Pt 22):5453-66