NUP84/YDL116W Summary Help

NUP84 BASIC INFORMATION

Standard Name NUP84
Systematic Name YDL116W
Feature Type ORF, Verified
Description Subunit of the nuclear pore complex (NPC), forms a subcomplex with Nup85p, Nup120p, Nup145p-C, Sec13p, and Seh1p that plays a role in nuclear mRNA export and NPC biogenesis (1 and see Summary Paragraph)
Name Description NUclear Pore
GO Annotations All NUP84 GO evidence and references
    View Computational GO annotations for NUP84
Molecular Function
Manually curated
Biological Process
Manually curated
High-throughput
Cellular Component
Manually curated
Mutant Phenotype All NUP84 Phenotype details and references
Classical genetics
null
Large-scale survey
null
Interactions NUP84 All interactions details and references
364 total interaction(s) for 216 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 72
  • Affinity Capture-Western: 20
  • Co-purification: 11
  • FRET: 2
  • Reconstituted Complex: 11
  • Two-hybrid: 5
Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Rescue: 1
  • Phenotypic Enhancement: 175
  • Phenotypic Suppression: 32
  • Synthetic Growth Defect: 11
  • Synthetic Haploinsufficiency: 1
  • Synthetic Lethality: 22
Sequence Information
ChrIV:251566 to 253746 | ORF Map | GBrowse
Gbrowse
Last Update Coordinates: 2004-02-11 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..2181 251566..253746 2004-02-11 1996-07-31
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | UniProtKB
Primary SGDIDS000002274

NUP84 RESOURCES

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SGD ORF map
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  • Localization Resources
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Click on histogram for expression summary
Expression Summary histogram

ADDITIONAL INFORMATION for NUP84

SUMMARY PARAGRAPH for NUP84

NUP84 encodes a nuclear pore protein homologous to the rat nucleoporin Nup107p (2, 3). Transport of macromolecules between the nucleus and the cytoplasm of eukaryotic cells occurs through the nuclear pore complex (NPC), a large macromolecular complex that spans the nuclear envelope (reviewed in 3, 4, 5, 6). The structure of the vertebrate NPC has been studied extensively; recent reviews include 7, 8, 9, and 10. The yeast NPC shares several features with the vertebrate NPC, despite being smaller and less elaborate (11, 12). Many yeast nuclear pore proteins, or nucleoporins, have been identified by a variety of genetic approaches (reviewed in 3, 4, 13, 14, 15). NUP84 was identified using an allele that is synthetically lethal with a temperature sensitive allele of NSP1 (2). Nup84p is part of subcomplex within the NPC that also includes Sec13p, Seh1p, Nup85p, and Nup120p (2, 3). The nup84 null mutant is viable but temperature sensitive. At the restrictive temperature the mutant shows altered distribution of NPCs and accumulates poly(A) RNA in the nucleus, while nuclear proteins are correctly localized. The seh1 nup84 double null is inviable (2). NUP84 also shows genetic interactions with a sporulation factor, SPO7, and a novel gene, NEM1, which both appear to be involved in nuclear envelope biogenesis (16).

Last updated: 1999-08-03

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for NUP84]

1) Siniossoglou S, et al.  (2000) Structure and assembly of the Nup84p complex. J Cell Biol 149(1):41-54
2) Siniossoglou S, et al.  (1996) A novel complex of nucleoporins, which includes Sec13p and a Sec13p homolog, is essential for normal nuclear pores. Cell 84(2):265-75
3) Fabre E and Hurt E  (1997) Yeast genetics to dissect the nuclear pore complex and nucleocytoplasmic trafficking. Annu Rev Genet 31:277-313
4) Wente SR, et al.  (1997) "The nucleus and nucleocytoplasmic transport in Saccharomyces cerevisiae." Pp. 471-546 in The Molecular and Cellular Biology of the Yeast Saccharomyces: Cell Cycle and Cell Biology, edited by Pringle JR, Broach JR and Jones EW. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press
5) Pemberton LF, et al.  (1998) Transport routes through the nuclear pore complex. Curr Opin Cell Biol 10(3):392-9
6) Izaurralde E and Adam S  (1998) Transport of macromolecules between the nucleus and the cytoplasm. RNA 4(4):351-64
7) Hinshaw JE  (1994) Architecture of the nuclear pore complex and its involvement in nucleocytoplasmic transport. Biochem Pharmacol 47(1):15-20
8) Pante N and Aebi U  (1996) Molecular dissection of the nuclear pore complex. Crit Rev Biochem Mol Biol 31(2):153-99
9) Davis LI  (1995) The nuclear pore complex. Annu Rev Biochem 64:865-96
10) Pante N and Aebi U  (1994) Toward the molecular details of the nuclear pore complex. J Struct Biol 113(3):179-89
11) Rout MP and Blobel G  (1993) Isolation of the yeast nuclear pore complex. J Cell Biol 123(4):771-83
12) Yang Q, et al.  (1998) Three-dimensional architecture of the isolated yeast nuclear pore complex: functional and evolutionary implications. Mol Cell 1(2):223-34
13) Doye V and Hurt E  (1997) From nucleoporins to nuclear pore complexes. Curr Opin Cell Biol 9(3):401-11
14) Doye V and Hurt EC  (1995) Genetic approaches to nuclear pore structure and function. Trends Genet 11(6):235-41
15) Newmeyer DD  (1993) The nuclear pore complex and nucleocytoplasmic transport. Curr Opin Cell Biol 5(3):395-407
16) Siniossoglou S, et al.  (1998) A novel complex of membrane proteins required for formation of a spherical nucleus. EMBO J 17(22):6449-64