IWR1/YDL115C Summary Help

Standard Name IWR1 1
Systematic Name YDL115C
Feature Type ORF, Verified
Description RNA polymerase II transport factor, conserved from yeast to humans; also has a role in transporting RNA polymerase III into the nucleus; interacts with most of the RNAP II subunits; nucleo-cytoplasmic shuttling protein; deletion causes hypersensitivity to K1 killer toxin; protein increases in abundance and relocalizes from nucleus to cytoplasm upon DNA replication stress (1, 2, 3, 4, 5, 6)
Name Description Interacts With RNA polymerase II 2
Chromosomal Location
ChrIV:255126 to 253995 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All IWR1 GO evidence and references
  View Computational GO annotations for IWR1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 1 genes
Resources
Classical genetics
null
Large-scale survey
null
overexpression
Resources
281 total interaction(s) for 215 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 51
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 2
  • Biochemical Activity: 4
  • Co-localization: 2
  • Reconstituted Complex: 1

Genetic Interactions
  • Negative Genetic: 151
  • Phenotypic Enhancement: 2
  • Phenotypic Suppression: 13
  • Positive Genetic: 28
  • Synthetic Growth Defect: 6
  • Synthetic Haploinsufficiency: 1
  • Synthetic Lethality: 11
  • Synthetic Rescue: 7

Resources
Expression Summary
histogram
Resources
Length (a.a.) 353
Molecular Weight (Da) 40,640
Isoelectric Point (pI) 4.43
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIV:255126 to 253995 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2007-07-10 | Sequence: 2007-07-10
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..82 255126..255045 2007-07-10 2007-07-10
Intron 83..152 255044..254975 2007-07-10 2007-07-10
CDS 153..1132 254974..253995 2007-07-10 2007-07-10
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002273
References cited on this page View Complete Literature Guide for IWR1
1) Page N, et al.  (2003) A Saccharomyces cerevisiae genome-wide mutant screen for altered sensitivity to K1 killer toxin. Genetics 163(3):875-94
2) Krogan NJ, et al.  (2006) Global landscape of protein complexes in the yeast Saccharomyces cerevisiae. Nature 440(7084):637-43
3) Peiro-Chova L and Estruch F  (2009) The yeast RNA polymerase II-associated factor Iwr1p is involved in the basal and regulated transcription of specific genes. J Biol Chem 284(42):28958-67
4) Czeko E, et al.  (2011) Iwr1 Directs RNA Polymerase II Nuclear Import. Mol Cell 42(2):261-6
5) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
6) Minaker SW, et al.  (2013) Biogenesis of RNA Polymerases II and III Requires the Conserved GPN Small GTPases in Saccharomyces cerevisiae. Genetics 193(3):853-64