KIN28/YDL108W Summary Help

Standard Name KIN28 1
Systematic Name YDL108W
Feature Type ORF, Verified
Description Serine/threonine protein kinase, subunit of transcription factor TFIIH; involved in transcription initiation at RNA polymerase II promoters; phosphorylates Ser5 residue of the PolII C-terminal domain (CTD) at gene promoters; relocalizes to the cytosol in response to hypoxia (2, 3, 4, 5)
Name Description protein KINase
Chromosomal Location
ChrIV:267698 to 268699 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All KIN28 GO evidence and references
  View Computational GO annotations for KIN28
Molecular Function
Manually curated
High-throughput
Biological Process
Manually curated
High-throughput
Cellular Component
Manually curated
Regulators 3 genes
Resources
Classical genetics
conditional
null
Large-scale survey
null
reduction of function
Resources
129 total interaction(s) for 79 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 14
  • Affinity Capture-Western: 11
  • Biochemical Activity: 19
  • Co-crystal Structure: 1
  • Co-localization: 2
  • Co-purification: 8
  • Far Western: 2
  • Reconstituted Complex: 21
  • Two-hybrid: 7

Genetic Interactions
  • Dosage Lethality: 2
  • Dosage Rescue: 8
  • Negative Genetic: 6
  • Positive Genetic: 7
  • Synthetic Growth Defect: 6
  • Synthetic Lethality: 13
  • Synthetic Rescue: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 306
Molecular Weight (Da) 35,247
Isoelectric Point (pI) 6.87
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIV:267698 to 268699 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2004-02-11 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..28 267698..267725 2004-02-11 1996-07-31
Intron 29..109 267726..267806 2004-02-11 1996-07-31
CDS 110..1002 267807..268699 2004-02-11 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002266
References cited on this page View Complete Literature Guide for KIN28
1) Simon M, et al.  (1986) KIN28, a yeast split gene coding for a putative protein kinase homologous to CDC28. EMBO J 5(10):2697-701
2) Rodriguez CR, et al.  (2000) Kin28, the TFIIH-associated carboxy-terminal domain kinase, facilitates the recruitment of mRNA processing machinery to RNA polymerase II. Mol Cell Biol 20(1):104-12
3) Keogh MC, et al.  (2002) Kin28 is found within TFIIH and a Kin28-Ccl1-Tfb3 trimer complex with differential sensitivities to T-loop phosphorylation. Mol Cell Biol 22(5):1288-97
4) Akhtar MS, et al.  (2009) TFIIH kinase places bivalent marks on the carboxy-terminal domain of RNA polymerase II. Mol Cell 34(3):387-93
5) Ghosh Dastidar R, et al.  (2012) The nuclear localization of SWI/SNF proteins is subjected to oxygen regulation. Cell Biosci 2(1):30