KIN28/YDL108W Summary

KIN28 BASIC INFORMATION

Standard Name	KIN28 1
Systematic Name	YDL108W
Feature Type	ORF, Verified
Description	Serine/threonine protein kinase, subunit of the transcription factor TFIIH; involved in transcription initiation at RNA polymerase II promoters (2, 3)
Name Description	protein KINase

GO Annotations	All KIN28 GO evidence and references
	View Computational GO annotations for KIN28
Molecular Function
Manually curated	NOT cyclin-dependent protein kinase activating kinase activity (IDA, IMP) cyclin-dependent protein kinase activity (TAS) general RNA polymerase II transcription factor activity (TAS) RNA polymerase II carboxy-terminal domain kinase activity (IDA, IMP, ISS)
Biological Process
Manually curated	phosphorylation of RNA polymerase II C-terminal domain (IMP) positive regulation of transcription from RNA polymerase II promoter (IMP) protein amino acid phosphorylation (IDA, IMP) transcription initiation from RNA polymerase II promoter (TAS)
Cellular Component
Manually curated	holo TFIIH complex (IDA, IPI) TFIIK complex (IDA)

Mutant Phenotype	All KIN28 Phenotype details and references
Classical genetics
conditional	resistance to cordycepin: decreased
null	inviable
Large-scale survey
null	inviable

Interactions	KIN28 All interactions details and references
	85 total interaction(s) for 49 unique genes/features.
Physical Interactions	Affinity Capture-MS: 9 Affinity Capture-Western: 6 Biochemical Activity: 18 Co-localization: 2 Co-purification: 5 Far Western: 2 Reconstituted Complex: 1 Two-hybrid: 7
Genetic Interactions	Dosage Lethality: 2 Dosage Rescue: 5 Phenotypic Enhancement: 3 Phenotypic Suppression: 6 Synthetic Growth Defect: 4 Synthetic Lethality: 13 Synthetic Rescue: 2

Sequence Information

ChrIV:267698 to 268699 | |

Last Update

Coordinates: 2004-02-11 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..28	267698..267725	2004-02-11	1996-07-31
Intron	29..109	267726..267806	2004-02-11	1996-07-31
CDS	110..1002	267807..268699	2004-02-11	1996-07-31

External Links	All Associated Seq \| E.C. \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| UniProtKB

Primary SGDID	S000002266

KIN28 RESOURCES

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SGD ORF map	GBrowse

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Expression Summary

ADDITIONAL INFORMATION for KIN28

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for KIN28]

1)	Simon M, et al. (1986) KIN28, a yeast split gene coding for a putative protein kinase homologous to CDC28. EMBO J 5(10):2697-701
2)	Rodriguez CR, et al. (2000) Kin28, the TFIIH-associated carboxy-terminal domain kinase, facilitates the recruitment of mRNA processing machinery to RNA polymerase II. Mol Cell Biol 20(1):104-12
3)	Keogh MC, et al. (2002) Kin28 is found within TFIIH and a Kin28-Ccl1-Tfb3 trimer complex with differential sensitivities to T-loop phosphorylation. Mol Cell Biol 22(5):1288-97

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