PHO2/YDL106C Summary Help

PHO2 BASIC INFORMATION

Standard Name PHO2
Systematic Name YDL106C
Alias BAS2 , GRF10
Feature Type ORF, Verified
Description Homeobox transcription factor; regulatory targets include genes involved in phosphate metabolism; binds cooperatively with Pho4p to the PHO5 promoter; phosphorylation of Pho2p facilitates interaction with Pho4p (1, 2, 3 and see Summary Paragraph)
Also known as: phoB 4
Name Description PHOsphate metabolism
GO Annotations All PHO2 GO evidence and references
    View Computational GO annotations for PHO2
Molecular Function
Manually curated
High-throughput
Biological Process
Manually curated
Cellular Component
Manually curated
Regulatory Role
Binding motifs PHO2 Transcription Factor Binding Sites and References
  • SGTGCGSYGYG
Regulatory modules predicted: stressResponse ( 571 )
Mutant Phenotype All PHO2 Phenotype details and references
Classical genetics
null
reduction of function
Large-scale survey
null
Interactions PHO2 All interactions details and references
56 total interaction(s) for 32 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 16
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 4
  • Biochemical Activity: 1
  • Reconstituted Complex: 14
  • Two-hybrid: 11
Genetic Interactions
  • Dosage Rescue: 1
  • Phenotypic Enhancement: 1
  • Phenotypic Suppression: 3
  • Synthetic Growth Defect: 3
  • Synthetic Lethality: 1
Sequence Information
ChrIV:271901 to 270222 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Genetic position: -58.01 cM
Last Update Coordinates: 2004-02-11 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..1680 271901..270222 2004-02-11 1996-07-31
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | UniProtKB
Primary SGDIDS000002264

PHO2 RESOURCES

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SGD ORF mapGBrowse
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  • Localization Resources
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Click on histogram for expression summary
Expression Summary histogram

ADDITIONAL INFORMATION for PHO2

NOMENCLATURE CONFLICT NOTE

NameRelevanceDescription
OLI1Nomenclature conflictPHO2 has been used to refer to both PHO2/YDL106C, which encodes a transcription factor, and OLI1/Q0130, the mitochondrial gene encoding an ATP synthase subunit.

SUMMARY PARAGRAPH for PHO2

PHO2, also known as BAS2 or GRF10, encodes a homeodomain transcriptional activator that activates transcription in a combinatorial manner (2,1). Pho2p is required to express genes in several different pathways such as purine nucleotide biosynthesis, histidine biosynthesis, and phosphate utilization (5). Genes that are known to be regulated by Pho2p include PHO5, PHO81, HIS4, CYC1, TRP4, HO, ADE1, ADE2, ADE5,7 and ADE8 (3). Although purified Pho2p has been shown in vitro to have low affinity DNA binding activity, in vivo, it activates transcription along with one of at least three distinct partner proteins: the zinc finger protein Swi5p (6), the basic-helix-loop-helix factor Pho4p (7), and Bas1p, a myb-like activator (5). Pho2p and Pho4p cooperatively bind to the promoter site of PHO5 (which encodes for a secreted acid phosphatase) and are required for PHO5 expression when cells are starved of phosphate (8). Pho2p and Swi5p together activate HO, while Pho2p and Bas1p activate genes in the purine and histidine biosynthesis pathways (8). Pho2p is phosphorylated by cdc2/CDC28-type kinase at the 'SPIK' site (amino acids 230-233). This phosphorylation of Pho2p facilitates the interaction between Pho2p and Pho4p which is required for PHO5 expression under low phosphate conditions. Deletion and point mutation analyses have revealed that the homeodomain (amino acids 77-136) is necessary but not sufficient for cooperative DNA binding, and that a large C-terminal region (amino acids 112-404), including the Pho80p homologous region (amino acids 241-258), is required for interaction with its partners Swi5p, Pho4p, and Bas1p (3).

Last updated: 2003-03-13

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for PHO2]

1) Lenburg ME and O'Shea EK  (1996) Signaling phosphate starvation. Trends Biochem Sci 21(10):383-7
2) Berben G, et al.  (1988) Studies on the structure, expression and function of the yeast regulatory gene PHO2. Gene 66(2):307-12
3) Liu C, et al.  (2000) Regulation of the yeast transcriptional factor PHO2 activity by phosphorylation. J Biol Chem 275(41):31972-8
4) To-E A, et al.  (1973) Isolation and characterization of acid phosphatase mutants in Saccharomyces cerevisiae. J Bacteriol 113(2):727-38
5) Daignan-Fornier B and Fink GR  (1992) Coregulation of purine and histidine biosynthesis by the transcriptional activators BAS1 and BAS2. Proc Natl Acad Sci U S A 89(15):6746-50
6) Brazas RM and Stillman DJ  (1993) The Swi5 zinc-finger and Grf10 homeodomain proteins bind DNA cooperatively at the yeast HO promoter. Proc Natl Acad Sci U S A 90(23):11237-41
7) Vogel K, et al.  (1989) The two positively acting regulatory proteins PHO2 and PHO4 physically interact with PHO5 upstream activation regions. Mol Cell Biol 9(5):2050-7
8) Bhoite LT, et al.  (2002) Mutations in the pho2 (bas2) transcription factor that differentially affect activation with its partner proteins bas1, pho4, and swi5. J Biol Chem 277(40):37612-8