QRI7/YDL104C Summary Help

Standard Name QRI7 1
Systematic Name YDL104C
Feature Type ORF, Verified
Description Protein involved in threonylcarbamoyl adenosine biosynthesis; Sua5p and Qri7p are necessary and sufficient for RNA t6A modification in vitro; highly conserved mitochondrial protein; essential for t6A modification of mitochondrial tRNAs that decode ANN codons; similar to Kae1p and E. coli YgjD, both of which are also required for tRNA t6A modification; when directed to the cytoplasm, complements the essential function of Kae1p in the KEOPS complex (2, 3, 4, 5, 6, 7)
Chromosomal Location
ChrIV:274876 to 273653 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All QRI7 GO evidence and references
  View Computational GO annotations for QRI7
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Regulators 4 genes
Classical genetics
Large-scale survey
36 total interaction(s) for 35 unique genes/features.
Genetic Interactions
  • Negative Genetic: 34
  • Positive Genetic: 2

Expression Summary
Length (a.a.) 407
Molecular Weight (Da) 45,543
Isoelectric Point (pI) 7.67
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrIV:274876 to 273653 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 2004-02-11 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..1224 274876..273653 2004-02-11 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002262
References cited on this page View Complete Literature Guide for QRI7
1) Simon M, et al.  (1994) Sequence of the PHO2-POL3 (CDC2) region of chromosome IV of Saccharomyces cerevisiae. Yeast 10(12):1653-6
2) Saiz JE, et al.  (1996) The sequence of a 20.3 kb DNA fragment from the left arm of Saccharomyces cerevisiae chromosome IV contains the KIN28, MSS2, PHO2, POL3 and DUN1 genes, and six new open reading frames. Yeast 12(10B Suppl):1077-84
3) Jonson L, et al.  (2004) Enhanced peptide secretion by gene disruption of CYM1, a novel protease in Saccharomyces cerevisiae. Eur J Biochem 271(23-24):4788-97
4) Reinders J, et al.  (2006) Toward the complete yeast mitochondrial proteome: multidimensional separation techniques for mitochondrial proteomics. J Proteome Res 5(7):1543-54
5) Oberto J, et al.  (2009) Qri7/OSGEPL, the mitochondrial version of the universal Kae1/YgjD protein, is essential for mitochondrial genome maintenance. Nucleic Acids Res 37(16):5343-52
6) Srinivasan M, et al.  (2011) The highly conserved KEOPS/EKC complex is essential for a universal tRNA modification, t6A. EMBO J 30(5):873-81
7) Wan LC, et al.  (2013) Reconstitution and characterization of eukaryotic N6-threonylcarbamoylation of tRNA using a minimal enzyme system. Nucleic Acids Res 41(12):6332-46