GET3/YDL100C Summary

GET3/YDL100C Summary

Standard Name	GET3 1
Systematic Name	YDL100C
Alias	ARR4 2
Feature Type	ORF, Verified
Description	Guanine nucleotide exchange factor for Gpa1p; amplifies G protein signaling; subunit of the GET complex, which is involved in Golgi to ER trafficking and insertion of proteins into the ER membrane; has low-level ATPase activity; protein abundance increases in response to DNA replication stress (1, 2, 3, 4, 5)
Name Description	Guided Entry of Tailanchored proteins 4

Chromosomal Location	ChrIV:283176 to 282112 \| ORF Map \| GBrowse
	Note: this feature is encoded on the Crick strand.

Gene Ontology Annotations All GET3 GO evidence and references

	View Computational GO annotations for GET3
Molecular Function
Manually curated	ATPase activity (IDA) guanyl-nucleotide exchange factor activity (IDA)
Biological Process
Manually curated	pheromone-dependent signal transduction involved in conjugation with cellular fusion (IMP) posttranslational protein targeting to membrane (IDA) protein insertion into ER membrane (IMP) response to heat (IMP) response to metal ion (IMP) retrograde vesicle-mediated transport, Golgi to ER (IDA, IGI, IMP)
Cellular Component
Manually curated	GET complex (IPI)
High-throughput	endoplasmic reticulum (IDA)

Mutant phenotypes All GET3 Phenotype evidence and references

Classical genetics
null	heat sensitivity: increased mating efficiency: decreased mating response: decreased metal resistance: decreased Hsp104p accumulation: increased resistance to copper(2+) sulfate: decreased resistance to hygromycin B: decreased resistance to paromomycin: decreased viable
overexpression	mating response: increased
Large-scale survey
null	acid pH resistance: decreased competitive fitness: decreased dessication resistance: decreased haploinsufficient heat sensitivity: increased protein transport: abnormal resistance to cycloheximide: increased resistance to camptothecin: increased resistance to caffeine: increased resistance to mycophenolic acid: decreased resistance to ethanol: decreased resistance to sirolimus: decreased resistance to acetic acid: decreased resistance to bleomycin: decreased resistance to fenpropimorph: decreased resistance to fluconazole: decreased resistance to hydroxyurea: decreased resistance to L-1,4-dithiothreitol: decreased resistance to clioquinol: decreased resistance to BPS: decreased resistance to methyl methanesulfonate: decreased resistance to hygromycin B: decreased starvation resistance: decreased viable
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All GET3 Interaction evidence and references

	481 total interaction(s) for 224 unique genes/features.
Physical Interactions	Affinity Capture-MS: 26 Affinity Capture-RNA: 2 Affinity Capture-Western: 12 Biochemical Activity: 1 Co-crystal Structure: 11 Co-fractionation: 2 Co-purification: 1 PCA: 3 Reconstituted Complex: 10 Two-hybrid: 39
Genetic Interactions	Dosage Growth Defect: 1 Negative Genetic: 222 Phenotypic Enhancement: 68 Phenotypic Suppression: 32 Positive Genetic: 35 Synthetic Growth Defect: 8 Synthetic Lethality: 5 Synthetic Rescue: 3
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder \| YeastRC Two-Hybrid

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All GET3 Protein evidence and references

Localization	YeastRC \| Organelle DB (UMich) \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| CGD Homologs \| CandidaDB Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrIV:283176 to 282112 | |

Note: this feature is encoded on the Crick strand.

SGD ORF map

Last Update

Coordinates: 2004-02-11 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..1065	283176..282112	2004-02-11	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| E.C. \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000002258

References cited on this page View Complete Literature Guide for GET3

1)	Schuldiner M, et al. (2005) Exploration of the function and organization of the yeast early secretory pathway through an epistatic miniarray profile. Cell 123(3):507-19
2)	Shen J, et al. (2003) The Saccharomyces cerevisiae Arr4p is involved in metal and heat tolerance. Biometals 16(3):369-78
3)	Lee MJ and Dohlman HG (2008) Coactivation of G protein signaling by cell-surface receptors and an intracellular exchange factor. Curr Biol 18(3):211-5
4)	Schuldiner M, et al. (2008) The GET complex mediates insertion of tail-anchored proteins into the ER membrane. Cell 134(4):634-45
5)	Tkach JM, et al. (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76