Standard Name	RPN6 1
Systematic Name	YDL097C
Alias	NAS4
Feature Type	ORF, Verified
Description	Essential, non-ATPase regulatory subunit of the 26S proteasome lid; required for the assembly and activity of the 26S proteasome; the human homolog (S9 protein) partially rescues Rpn6p depletion; protein abundance increases in response to DNA replication stress (2, 3, 4)
Name Description	Regulatory Particle Non-ATPase 1

Chromosomal Location	ChrIV:286695 to 285391 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.

	View Computational GO annotations for RPN6
Molecular Function
Manually curated	structural molecule activity (IMP)
Biological Process
Manually curated	proteasome assembly (IMP) ubiquitin-dependent protein catabolic process (IMP)
Cellular Component
Manually curated	proteasome regulatory particle, lid subcomplex (IDA) proteasome storage granule (IDA)

Classical genetics
conditional	colony sectoring: increased resistance to bleomycin: decreased resistance to hydroxyurea: decreased
Large-scale survey
conditional	chromosome/plasmid maintenance: abnormal colony sectoring: increased heat sensitivity: increased
null	competitive fitness: decreased haploinsufficient inviable resistance to 2-phenyl-3-nitroso-imidazo[1,2-a]pyridine: decreased resistance to clioquinol: decreased
reduction of function	competitive fitness: decreased telomere length: decreased
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	300 total interaction(s) for 133 unique genes/features.
Physical Interactions	Affinity Capture-MS: 152 Affinity Capture-RNA: 2 Affinity Capture-Western: 10 Co-purification: 18 PCA: 2 Reconstituted Complex: 4 Two-hybrid: 3
Genetic Interactions	Negative Genetic: 70 Positive Genetic: 33 Synthetic Haploinsufficiency: 1 Synthetic Lethality: 5
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder | YeastRC Two-Hybrid

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | Organelle DB (UMich) | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | CGD Homologs | CandidaDB Homologs | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrIV:286695 to 285391 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.
Last Update	Coordinates: 2004-02-11 | Sequence: 1996-07-31
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..1305 286695..285391 2004-02-11 1996-07-31
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI1631 S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CEN.PK113-7D S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FL100 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000002255