RPN6/YDL097C Summary Help

Standard Name RPN6 1
Systematic Name YDL097C
Alias NAS4
Feature Type ORF, Verified
Description Essential, non-ATPase regulatory subunit of the 26S proteasome lid; required for the assembly and activity of the 26S proteasome; the human homolog (S9 protein) partially rescues Rpn6p depletion; protein abundance increases in response to DNA replication stress (2, 3, 4)
Name Description Regulatory Particle Non-ATPase 1
Chromosomal Location
ChrIV:286695 to 285391 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All RPN6 GO evidence and references
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 6 genes
Resources
Classical genetics
conditional
Large-scale survey
conditional
null
reduction of function
Resources
333 total interaction(s) for 156 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 158
  • Affinity Capture-RNA: 4
  • Affinity Capture-Western: 10
  • Co-purification: 18
  • PCA: 2
  • Reconstituted Complex: 4
  • Two-hybrid: 3

Genetic Interactions
  • Negative Genetic: 88
  • Positive Genetic: 40
  • Synthetic Haploinsufficiency: 1
  • Synthetic Lethality: 5

Resources
Expression Summary
histogram
Resources
Length (a.a.) 434
Molecular Weight (Da) 49,773
Isoelectric Point (pI) 6.22
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIV:286695 to 285391 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2004-02-11 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1305 286695..285391 2004-02-11 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002255
References cited on this page View Complete Literature Guide for RPN6
1) Finley D, et al.  (1998) Unified nomenclature for subunits of the Saccharomyces cerevisiae proteasome regulatory particle. Trends Biochem Sci 23(7):244-5
2) Saito A, et al.  (1997) cDNA cloning and functional analysis of p44.5 and p55, two regulatory subunits of the 26S proteasome. Gene 203(2):241-50
3) Santamaria PG, et al.  (2003) Rpn6p, a proteasome subunit from Saccharomyces cerevisiae, is essential for the assembly and activity of the 26 S proteasome. J Biol Chem 278(9):6687-95
4) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76