SRP14/YDL092W Summary Help

Standard Name SRP14
Systematic Name YDL092W
Feature Type ORF, Verified
Description Signal recognition particle (SRP) subunit; interacts with the RNA component of SRP to form the Alu domain, which is the region of SRP responsible for arrest of nascent chain elongation during membrane targeting; homolog of mammalian SRP14 (1, 2, 3 and see Summary Paragraph)
Name Description Signal Recognition Particle
Chromosomal Location
ChrIV:292781 to 293221 | ORF Map | GBrowse
Gene Ontology Annotations All SRP14 GO evidence and references
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 3 genes
Classical genetics
Large-scale survey
48 total interaction(s) for 24 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 31
  • Affinity Capture-RNA: 6
  • Co-purification: 2
  • PCA: 7
  • Reconstituted Complex: 1
  • Two-hybrid: 1

Expression Summary
Length (a.a.) 146
Molecular Weight (Da) 16,430
Isoelectric Point (pI) 10.51
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrIV:292781 to 293221 | ORF Map | GBrowse
Last Update Coordinates: 2004-02-11 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..441 292781..293221 2004-02-11 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002250

The signal recognition particle (SRP) is an abundant and conserved ribonucleoprotein necessary for targeting proteins to the endoplasmic reticulum membrane (4). SRP in eukaryotes contains six subunits and a 7S RNA molecule; in S. cerevisiae the subunits are encoded by SRP14, SRP21, SRP68, SRP72, SEC65, and SRP54, and the RNA (termed scR1) is encoded by SCR1 (4, 3). With the exception of Srp54p, the proteins and RNA assemble into a core complex in the nucleus; this particle is exported to the cytoplasm where Srp54p joins to form the complete complex (5). Sec65p is required for association of Srp54p with the SRP particle (6). Loss of any of the SRP components causes a slow growth phenotype and loss of SRP-mediated translocation, but not cell death, indicating that the signal recognition particle is not essential in yeast and SRP-independent translocation can occur (4, 3).

The first step of SRP-mediated cotranslational targeting is interaction between SRP and the ribosome nascent chain complex (RNC), which is comprised of the translating ribosome and the emerging nascent protein. SRP interacts with the RNC through the N-terminal hydrophobic signal sequence of the nascent protein. SRP then directs the RNC to the ER membrane via interaction between SRP and a signal receptor complex (SR), encoded by SRP101 and SRP102. Finally, the RNC is transferred to the translocon, a protein-conducting membrane channel, and SRP and the SR dissociate. GTP binding by both SRP (via the Srp54p subunit) and the SR is critical for their interaction, and GTP hydrolysis facilitates their dissociation (reviewed in 7, and see 7 for more details).

Last updated: 2008-08-11 Contact SGD

References cited on this page View Complete Literature Guide for SRP14
1) Strub K, et al.  (1999) The Alu domain homolog of the yeast signal recognition particle consists of an Srp14p homodimer and a yeast-specific RNA structure. RNA 5(10):1333-47
2) Mason N, et al.  (2000) Elongation arrest is a physiologically important function of signal recognition particle. EMBO J 19(15):4164-74
3) Brown JD, et al.  (1994) Subunits of the Saccharomyces cerevisiae signal recognition particle required for its functional expression. EMBO J 13(18):4390-400
4) Hann BC and Walter P  (1991) The signal recognition particle in S. cerevisiae. Cell 67(1):131-44
5) Ciufo LF and Brown JD  (2000) Nuclear export of yeast signal recognition particle lacking Srp54p by the Xpo1p/Crm1p NES-dependent pathway. Curr Biol 10(20):1256-64
6) Stirling CJ and Hewitt EW  (1992) The S. cerevisiae SEC65 gene encodes a component of yeast signal recognition particle with homology to human SRP19. Nature 356(6369):534-7
7) Wild K, et al.  (2004) SRP meets the ribosome. Nat Struct Mol Biol 11(11):1049-53