RAM1/YDL090C Summary Help

Standard Name RAM1 1
Systematic Name YDL090C
Alias DPR1 , FUS8 , SCG2 , SGP2 , STE16 2
Feature Type ORF, Verified
Description Beta subunit of the CAAX farnesyltransferase (FTase); this complex prenylates the a-factor mating pheromone and Ras proteins; required for the membrane localization of Ras proteins and a-factor; homolog of the mammalian FTase beta subunit (3, 4, 5, 6, 7)
Name Description RAS protein and A-factor Maturation 8
Chromosomal Location
ChrIV:296329 to 295034 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Genetic position: -33 cM
Gene Ontology Annotations All RAM1 GO evidence and references
  View Computational GO annotations for RAM1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 9 genes
Resources
Classical genetics
null
unspecified
Large-scale survey
null
overexpression
unspecified
Resources
52 total interaction(s) for 46 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 4
  • Affinity Capture-RNA: 3
  • Two-hybrid: 4

Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Lethality: 1
  • Dosage Rescue: 2
  • Negative Genetic: 27
  • Phenotypic Suppression: 2
  • Positive Genetic: 4
  • Synthetic Growth Defect: 1
  • Synthetic Lethality: 2
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 431
Molecular Weight (Da) 48,189
Isoelectric Point (pI) 6.35
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIV:296329 to 295034 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Genetic position: -33 cM
Last Update Coordinates: 2004-02-11 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1296 296329..295034 2004-02-11 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002248
References cited on this page View Complete Literature Guide for RAM1
1) Schafer WR, et al.  (1990) Enzymatic coupling of cholesterol intermediates to a mating pheromone precursor and to the ras protein. Science 249(4973):1133-9
2) Wilson KL and Herskowitz I  (1987) STE16, a new gene required for pheromone production by a cells of Saccharomyces cerevisiae. Genetics 115(3):441-9
3) Trueblood CE, et al.  (1997) Substrate specificity determinants in the farnesyltransferase beta-subunit. Proc Natl Acad Sci U S A 94(20):10774-9
4) Gomez R, et al.  (1993) Purified yeast protein farnesyltransferase is structurally and functionally similar to its mammalian counterpart. Biochem J 289 ( Pt 1)():25-31
5) He B, et al.  (1991) RAM2, an essential gene of yeast, and RAM1 encode the two polypeptide components of the farnesyltransferase that prenylates a-factor and Ras proteins. Proc Natl Acad Sci U S A 88(24):11373-7
6) Kohl NE, et al.  (1991) Structural homology among mammalian and Saccharomyces cerevisiae isoprenyl-protein transferases. J Biol Chem 266(28):18884-8
7) Kurth DD, et al.  (1997) Functional consequence of mutating conserved residues of the yeast farnesyl-protein transferase beta-subunit Ram1(Dpr1). Biochemistry 36(50):15932-9
8) Powers S, et al.  (1986) RAM, a gene of yeast required for a functional modification of RAS proteins and for production of mating pheromone a-factor. Cell 47(3):413-22