ASM4/YDL088C Summary Help

ASM4 BASIC INFORMATION

Standard Name ASM4
Systematic Name YDL088C
Alias NUP59
Feature Type ORF, Verified
Description Nuclear pore complex subunit, part of a subcomplex also containing Nup53p, Nup170p, and Pse1p (1 and see Summary Paragraph)
GO Annotations All ASM4 GO evidence and references
    View Computational GO annotations for ASM4
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Mutant Phenotype All ASM4 Phenotype details and references
Classical genetics
null
overexpression
Large-scale survey
null
overexpression
Interactions ASM4 All interactions details and references
54 total interaction(s) for 37 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 3
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 2
  • Biochemical Activity: 1
  • Co-purification: 1
  • PCA: 1
  • Reconstituted Complex: 4
  • Two-hybrid: 2
Genetic Interactions
  • Phenotypic Enhancement: 20
  • Phenotypic Suppression: 12
  • Synthetic Growth Defect: 2
  • Synthetic Lethality: 5
Sequence Information
ChrIV:300003 to 298417 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Last Update Coordinates: 2004-02-11 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..1587 300003..298417 2004-02-11 1996-07-31
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | UniProtKB
Primary SGDIDS000002246

ASM4 RESOURCES

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SGD ORF mapGBrowse
SGD ORF map
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  • Retrieve Sequences
  • Sequence Analysis Tools
  • Protein Info & Structure
  • Localization Resources
  • Interactions
  • Phenotype Resources
  • Maps & Displays
  • Comparison Resources
  • Functional Analysis

Click on histogram for expression summary
Expression Summary histogram

ADDITIONAL INFORMATION for ASM4

SUMMARY PARAGRAPH for ASM4

Asm4p is a nuclear pore protein that forms a complex with two other nucleoporins, Nup53p and Nup170p (1). Transport of macromolecules between the nucleus and the cytoplasm of eukaryotic cells occurs through the nuclear pore complex (NPC), a large macromolecular complex that spans the nuclear envelope (reviewed in 2, 3, 4, 5). The structure of the vertebrate NPC has been studied extensively; recent reviews include 6, 7, 8, and 9. The yeast NPC shares several features with the vertebrate NPC, despite being smaller and less elaborate (10, 11). Many yeast nuclear pore proteins, or nucleoporins, have been identified by a variety of genetic approaches (reviewed in 2, 3, 12, 13, 14). The Asm4p-containing complex also interacts with the karyopherin Pse1p (1). Lopez et al. (15) report that ASM4 is essential for vegetative growth in some strain backgrounds tested. Mutations in ASM4 also show genetic interactions with mutations in other nucleoporin genes (16, 1) and with POL3, which encodes a subunit of DNA polymerase delta (17). Asm4p is structurally similar to Nup53p, and similar protein sequences are found in several eukaryotes (1).

Last updated: 1999-08-03

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for ASM4]

1) Marelli M, et al.  (1998) Specific binding of the karyopherin Kap121p to a subunit of the nuclear pore complex containing Nup53p, Nup59p, and Nup170p. J Cell Biol 143(7):1813-30
2) Fabre E and Hurt E  (1997) Yeast genetics to dissect the nuclear pore complex and nucleocytoplasmic trafficking. Annu Rev Genet 31:277-313
3) Wente SR, et al.  (1997) "The nucleus and nucleocytoplasmic transport in Saccharomyces cerevisiae." Pp. 471-546 in The Molecular and Cellular Biology of the Yeast Saccharomyces: Cell Cycle and Cell Biology, edited by Pringle JR, Broach JR and Jones EW. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press
4) Pemberton LF, et al.  (1998) Transport routes through the nuclear pore complex. Curr Opin Cell Biol 10(3):392-9
5) Izaurralde E and Adam S  (1998) Transport of macromolecules between the nucleus and the cytoplasm. RNA 4(4):351-64
6) Hinshaw JE  (1994) Architecture of the nuclear pore complex and its involvement in nucleocytoplasmic transport. Biochem Pharmacol 47(1):15-20
7) Pante N and Aebi U  (1996) Molecular dissection of the nuclear pore complex. Crit Rev Biochem Mol Biol 31(2):153-99
8) Davis LI  (1995) The nuclear pore complex. Annu Rev Biochem 64:865-96
9) Pante N and Aebi U  (1994) Toward the molecular details of the nuclear pore complex. J Struct Biol 113(3):179-89
10) Rout MP and Blobel G  (1993) Isolation of the yeast nuclear pore complex. J Cell Biol 123(4):771-83
11) Yang Q, et al.  (1998) Three-dimensional architecture of the isolated yeast nuclear pore complex: functional and evolutionary implications. Mol Cell 1(2):223-34
12) Doye V and Hurt E  (1997) From nucleoporins to nuclear pore complexes. Curr Opin Cell Biol 9(3):401-11
13) Doye V and Hurt EC  (1995) Genetic approaches to nuclear pore structure and function. Trends Genet 11(6):235-41
14) Newmeyer DD  (1993) The nuclear pore complex and nucleocytoplasmic transport. Curr Opin Cell Biol 5(3):395-407
15) Lopez MC, et al.  (1998) Disruption of six Saccharomyces cerevisiae genes from chromosome IV and basic phenotypic analysis of deletion mutants. Yeast 14(13):1199-208
16) Tcheperegine SE, et al.  (1999) Topology and functional domains of the yeast pore membrane protein Pom152p. J Biol Chem 274(8):5252-8
17) Giot L, et al.  (1995) Suppressors of thermosensitive mutations in the DNA polymerase delta gene of Saccharomyces cerevisiae. Mol Gen Genet 246(2):212-22