THI3/YDL080C Summary Help

Standard Name THI3
Systematic Name YDL080C
Alias KID1 1
Feature Type ORF, Verified
Description Regulatory protein that binds Pdc2p and Thi2p transcription factors; activates thiamine biosynthesis transcription factors Pdc2p and Thi2p by binding to them, but releases and de-activates them upon binding to thiamine pyrophosphate (TPP), the end product of the pathway; has similarity to decarboxylases but enzymatic activity is not detected (2, 3, 4, 5)
Name Description THIamine metabolism
Chromosomal Location
ChrIV:312471 to 310642 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All THI3 GO evidence and references
  View Computational GO annotations for THI3
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 3 genes
Resources
Pathways
Classical genetics
null
reduction of function
Large-scale survey
null
Resources
70 total interaction(s) for 57 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 7
  • Affinity Capture-RNA: 1
  • Co-purification: 1
  • Reconstituted Complex: 2
  • Two-hybrid: 2

Genetic Interactions
  • Negative Genetic: 44
  • Phenotypic Enhancement: 3
  • Phenotypic Suppression: 2
  • Positive Genetic: 3
  • Synthetic Growth Defect: 3
  • Synthetic Lethality: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 609
Molecular Weight (Da) 68,366
Isoelectric Point (pI) 6.33
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIV:312471 to 310642 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2004-02-11 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1830 312471..310642 2004-02-11 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002238
References cited on this page View Complete Literature Guide for THI3
1) Dickinson JR, et al.  (2003) The catabolism of amino acids to long chain and complex alcohols in Saccharomyces cerevisiae. J Biol Chem 278(10):8028-34
2) Nosaka K, et al.  (1993) Isolation and characterization of a thiamin pyrophosphokinase gene, THI80, from Saccharomyces cerevisiae. J Biol Chem 268(23):17440-7
3) Nishimura H, et al.  (1992) A positive regulatory gene, THI3, is required for thiamine metabolism in Saccharomyces cerevisiae. J Bacteriol 174(14):4701-6
4) Nosaka K, et al.  (2008) Thiamin-dependent transactivation activity of PDC2 in Saccharomyces cerevisiae. FEBS Lett 582(29):3991-6
5) Nosaka K, et al.  (2012) Facilitated recruitment of Pdc2p, a yeast transcriptional activator, in response to thiamin starvation. FEMS Microbiol Lett 330(2):140-7