Standard Name	VAM6
Systematic Name	YDL077C
Alias	CVT4 , VPL18 1 , VPL22 2 , VPS39 1 , 3
Feature Type	ORF, Verified
Description	Vacuolar protein that plays a critical role in the tethering steps of vacuolar membrane fusion by facilitating guanine nucleotide exchange on small guanosine triphosphatase Ypt7p (4, 5)
Name Description	VAcuolar Morphogenesis

Chromosomal Location	ChrIV:320120 to 316971 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.

	View Computational GO annotations for VAM6
Molecular Function
Manually curated	contributes_to phosphatidylinositol binding (IDA) Rab guanyl-nucleotide exchange factor activity (IDA)
Biological Process
Manually curated	piecemeal microautophagy of nucleus (IMP) regulation of SNARE complex assembly (IDA) regulation of vacuole fusion, non-autophagic (IDA) vacuole fusion, non-autophagic (IDA, IMP) vacuole organization (IMP) vesicle-mediated transport (IMP)
Cellular Component
Manually curated	fungal-type vacuole (IDA) fungal-type vacuole membrane (IDA) HOPS complex (IPI)

Classical genetics
null	tellurium atom accumulation: increased endocytosis: abnormal killer toxin resistance: decreased metal resistance: decreased GFP-Osh1p accumulation: increased carboxypeptidase Y (Prc1p) modification: decreased resistance to monensin: decreased resistance to brefeldin A: decreased resistance to Papulacandin B: decreased resistance to calcium dichloride: decreased
reduction of function	vacuolar morphology: abnormal vacuolar transport: abnormal vegetative growth: decreased
Large-scale survey
null	acid pH resistance: decreased auxotrophy glutathione excretion: increased inositol excretion: increased competitive fitness: decreased dessication resistance: decreased heat sensitivity: increased ionic stress resistance: decreased metal resistance: decreased Cps1p (CPS) modification: decreased Pho8p (ALP) modification: decreased resistance to ethanol: decreased resistance to sorbic acid: decreased resistance to monensin: decreased resistance to PM02734: decreased resistance to dihydromotuporamine C: decreased resistance to caffeine: decreased resistance to sirolimus: decreased resistance to tunicamycin: decreased resistance to wortmannin: decreased resistance to pyrrolidine dithiocarbamate: decreased resistance to hygromycin B: decreased resistance to spermine: decreased resistance to mefloquine: decreased resistance to ciclopirox olamine: decreased resistance to clioquinol: decreased resistance to 5-fluorouracil: decreased resistance to calcium dichloride: decreased respiratory growth: absent sporulation: absent sporulation efficiency: decreased telomere length: decreased utilization of nitrogen source: absent viable
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	275 total interaction(s) for 143 unique genes/features.
Physical Interactions	Affinity Capture-MS: 20 Affinity Capture-Western: 36 Co-fractionation: 1 Co-purification: 12 Reconstituted Complex: 7 Two-hybrid: 28
Genetic Interactions	Dosage Lethality: 1 Dosage Rescue: 6 Negative Genetic: 126 Phenotypic Enhancement: 1 Positive Genetic: 19 Synthetic Growth Defect: 13 Synthetic Lethality: 2 Synthetic Rescue: 3
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrIV:320120 to 316971 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.
Last Update	Coordinates: 2004-02-11 | Sequence: 1996-07-31
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..3150 320120..316971 2004-02-11 1996-07-31
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI1631 S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CBS7960 S.c. Strain CEN.PK113-7D S.c. Strain CLIB215 S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain T73 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain Y10 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000002235