BDF2/YDL070W Summary Help

Standard Name BDF2 1, 2
Systematic Name YDL070W
Feature Type ORF, Verified
Description Protein involved in transcription initiation; acts at TATA-containing promoters; associates with the basal transcription factor TFIID; contains two bromodomains; corresponds to the C-terminal region of mammalian TAF1; redundant with Bdf1p; protein abundance increases in response to DNA replication stress; BDF2 has a paralog, BDF1, that arose from the whole genome duplication (2, 3, 4)
Name Description BromoDomain Factor 1, 2
Chromosomal Location
ChrIV:331024 to 332940 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All BDF2 GO evidence and references
  View Computational GO annotations for BDF2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 11 genes
Resources
Classical genetics
null
Large-scale survey
null
overexpression
Resources
107 total interaction(s) for 79 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 11
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 3
  • Biochemical Activity: 22
  • Reconstituted Complex: 1
  • Two-hybrid: 12

Genetic Interactions
  • Dosage Rescue: 2
  • Negative Genetic: 36
  • Phenotypic Enhancement: 8
  • Phenotypic Suppression: 3
  • Positive Genetic: 2
  • Synthetic Growth Defect: 4
  • Synthetic Lethality: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 638
Molecular Weight (Da) 72,512
Isoelectric Point (pI) 6.04
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIV:331024 to 332940 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1917 331024..332940 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002228
References cited on this page View Complete Literature Guide for BDF2
1) Casalone E, et al.  (1999) Disruption and phenotypic analysis of six novel genes from chromosome IV of Saccharomyces cerevisiae reveal YDL060w as an essential gene for vegetative growth. Yeast 15(15):1691-701
2) Matangkasombut O, et al.  (2000) Bromodomain factor 1 corresponds to a missing piece of yeast TFIID. Genes Dev 14(8):951-62
3) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
4) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76