RAD59/YDL059C Summary Help

Standard Name RAD59
Systematic Name YDL059C
Feature Type ORF, Verified
Description Protein involved DNA double-strand break repair; repairs breaks in DNA during vegetative growth via recombination and single-strand annealing; anneals complementary single-stranded DNA; forms nuclear foci upon DNA replication stress; required for loading of Rad52p to DSBs; paralog of Rad52p (1, 2, 3, 4, 5)
Name Description RADiation sensitive
Chromosomal Location
ChrIV:344953 to 344237 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All RAD59 GO evidence and references
  View Computational GO annotations for RAD59
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 5 genes
Resources
Classical genetics
null
Large-scale survey
null
Resources
158 total interaction(s) for 105 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 33
  • Affinity Capture-Western: 11
  • Co-localization: 1
  • Reconstituted Complex: 3
  • Two-hybrid: 4

Genetic Interactions
  • Dosage Lethality: 1
  • Dosage Rescue: 1
  • Negative Genetic: 20
  • Phenotypic Enhancement: 24
  • Phenotypic Suppression: 16
  • Positive Genetic: 3
  • Synthetic Growth Defect: 31
  • Synthetic Lethality: 7
  • Synthetic Rescue: 3

Resources
Expression Summary
histogram
Resources
Length (a.a.) 238
Molecular Weight (Da) 26,630
Isoelectric Point (pI) 9.1
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIV:344953 to 344237 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2004-02-11 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..717 344953..344237 2004-02-11 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002217
References cited on this page View Complete Literature Guide for RAD59
1) Petukhova G, et al.  (1999) Single strand DNA binding and annealing activities in the yeast recombination factor Rad59. J Biol Chem 274(48):33839-42
2) Davis AP and Symington LS  (2001) The yeast recombinational repair protein Rad59 interacts with Rad52 and stimulates single-strand annealing. Genetics 159(2):515-25
3) Symington LS  (2002) Role of RAD52 epistasis group genes in homologous recombination and double-strand break repair. Microbiol Mol Biol Rev 66(4):630-70, table of contents
4) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
5) Pannunzio NR, et al.  (2012) Rad59 regulates association of Rad52 with DNA double-strand breaks. Microbiologyopen 1(3):285-97