SIT4/YDL047W Summary Help

Standard Name SIT4 1
Systematic Name YDL047W
Feature Type ORF, Verified
Description Type 2A-related serine-threonine phosphatase that functions in the G1/S transition of the mitotic cycle; cytoplasmic and nuclear protein that modulates functions mediated by Pkc1p including cell wall and actin cytoskeleton organization (2, 3, 4, 5, 6)
Name Description Suppressor of Initiation of Transcription 1
Chromosomal Location
ChrIV:369771 to 370706 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All SIT4 GO evidence and references
  View Computational GO annotations for SIT4
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
High-throughput
Mutant phenotypes All SIT4 Phenotype evidence and references
Classical genetics
conditional
null
Large-scale survey
null
overexpression
repressible
Resources
interactions All SIT4 Interaction evidence and references
269 total interaction(s) for 161 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 96
  • Affinity Capture-Western: 25
  • Biochemical Activity: 2
  • Co-fractionation: 2
  • Co-purification: 2
  • PCA: 2
  • Reconstituted Complex: 2
Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Lethality: 1
  • Dosage Rescue: 38
  • Negative Genetic: 13
  • Phenotypic Enhancement: 10
  • Phenotypic Suppression: 13
  • Positive Genetic: 19
  • Synthetic Growth Defect: 5
  • Synthetic Lethality: 26
  • Synthetic Rescue: 12
Resources
Expression Summary
histogram
Resources
Protein Information All SIT4 Protein evidence and references
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIV:369771 to 370706 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..936 369771..370706 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002205
References cited on this page View Complete Literature Guide for SIT4
1) Arndt KT, et al.  (1989) A suppressor of a HIS4 transcriptional defect encodes a protein with homology to the catalytic subunit of protein phosphatases. Cell 56(4):527-37
2) Stark MJ  (1996) Yeast protein serine/threonine phosphatases: multiple roles and diverse regulation. Yeast 12(16):1647-75
3) Sutton A, et al.  (1991) The SIT4 protein phosphatase functions in late G1 for progression into S phase. Mol Cell Biol 11(4):2133-48
4) Zabrocki P, et al.  (2002) Protein phosphatase 2A on track for nutrient-induced signalling in yeast. Mol Microbiol 43(4):835-42
5) Angeles de la Torre-Ruiz M, et al.  (2002) Sit4 is required for proper modulation of the biological functions mediated by Pkc1 and the cell integrity pathway in Saccharomyces cerevisiae. J Biol Chem 277(36):33468-76
6) Huh WK, et al.  (2003) Global analysis of protein localization in budding yeast. Nature 425(6959):686-91