SLM3/YDL033C Summary Help

Standard Name SLM3 1
Systematic Name YDL033C
Alias MTO2 2 , MTU1 3
Feature Type ORF, Verified
Description tRNA-specific 2-thiouridylase; responsible for 2-thiolation of the wobble base of mitochondrial tRNAs; human ortholog is implicated in myoclonus epilepsy associated with ragged red fibers (MERRF) (2, 3)
Name Description Synthetic Lethal with Mss4 1
Chromosomal Location
ChrIV:393912 to 392659 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All SLM3 GO evidence and references
  View Computational GO annotations for SLM3
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 1 genes
Resources
Classical genetics
null
Large-scale survey
null
Resources
161 total interaction(s) for 149 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 2
  • Affinity Capture-RNA: 1
  • Biochemical Activity: 1

Genetic Interactions
  • Negative Genetic: 108
  • Phenotypic Enhancement: 2
  • Positive Genetic: 28
  • Synthetic Growth Defect: 9
  • Synthetic Lethality: 9
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 417
Molecular Weight (Da) 47,049
Isoelectric Point (pI) 8.68
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIV:393912 to 392659 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1254 393912..392659 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002191
References cited on this page View Complete Literature Guide for SLM3
1) Audhya A, et al.  (2004) Genome-wide lethality screen identifies new PI4,5P2 effectors that regulate the actin cytoskeleton. EMBO J 23(19):3747-57
2) Yan Q, et al.  (2005) Mutations in MTO2 related to tRNA modification impair mitochondrial gene expression and protein synthesis in the presence of a paromomycin resistance mutation in mitochondrial 15 S rRNA. J Biol Chem 280(32):29151-7
3) Umeda N, et al.  (2005) Mitochondria-specific RNA-modifying enzymes responsible for the biosynthesis of the wobble base in mitochondrial tRNAs. Implications for the molecular pathogenesis of human mitochondrial diseases. J Biol Chem 280(2):1613-24