GPD1/YDL022W Summary

GPD1/YDL022W Summary

Standard Name	GPD1 1, 2
Systematic Name	YDL022W
Alias	DAR1 3 , HOR1 4 , OSG1 5 , OSR5
Feature Type	ORF, Verified
Description	NAD-dependent glycerol-3-phosphate dehydrogenase; key enzyme of glycerol synthesis, essential for growth under osmotic stress; expression regulated by high-osmolarity glycerol response pathway; protein abundance increases in response to DNA replication stress; constitutively inactivated via phosphorylation by the protein kinases YPK1 and YPK2, dephosphorylation increases catalytic activity; GPD1 has a paralog, GPD2, that arose from the whole genome duplication (6, 7, 8, 9, 10)
Name Description	Glycerol-3-Phosphate Dehydrogenase 7

Chromosomal Location	ChrIV:411825 to 413000 \| ORF Map \| GBrowse

Gene Ontology Annotations All GPD1 GO evidence and references

	View Computational GO annotations for GPD1
Molecular Function
Manually curated	glycerol-3-phosphate dehydrogenase [NAD+] activity (IMP)
Biological Process
Manually curated	intracellular accumulation of glycerol (IMP) NADH oxidation (IMP)
Cellular Component
Manually curated	cytosol (IDA) nucleus (IDA) peroxisome (IDA)

Pathways	glycerol biosynthesis phosphatidate biosynthesis II (the glycerol-3-phosphate pathway) superpathway of phosphatidate biosynthesis

Mutant phenotypes All GPD1 Phenotype evidence and references

Classical genetics
null	glycerol accumulation: decreased ethanol accumulation: increased hyperosmotic stress resistance: decreased resistance to methotrexate: increased
overexpression	ethanol accumulation: decreased 2,3 butane-diol accumulation: increased acetoin accumulation: increased glycerol accumulation: increased acetate accumulation: increased
Large-scale survey
null	auxotrophy competitive fitness: decreased hyperosmotic stress resistance: decreased resistance to 2-coumaric acid: decreased resistance to mefloquine: decreased resistance to calcium dichloride: decreased resistance to tacrolimus (anhydrous) and tunicamycin: increased resistance to glucose: decreased resistance to 1,4-dithiothreitol and tacrolimus (anhydrous): increased vegetative growth: decreased rate viable
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All GPD1 Interaction evidence and references

	67 total interaction(s) for 51 unique genes/features.
Physical Interactions	Affinity Capture-MS: 12 Affinity Capture-RNA: 4 Biochemical Activity: 1 Two-hybrid: 1
Genetic Interactions	Dosage Growth Defect: 1 Dosage Rescue: 10 Negative Genetic: 21 Phenotypic Enhancement: 4 Phenotypic Suppression: 1 Positive Genetic: 2 Synthetic Growth Defect: 7 Synthetic Lethality: 2 Synthetic Rescue: 1
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All GPD1 Protein evidence and references

Localization	YeastRC \| Organelle DB (UMich) \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| AspGD Homologs \| CGD Homologs \| CandidaDB Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrIV:411825 to 413000 | |

SGD ORF map

Last Update

Coordinates: 2011-02-03 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..1176	411825..413000	2011-02-03	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| E.C. \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000002180

References cited on this page View Complete Literature Guide for GPD1

1)	Andre, B., et al., (1995) The sequence of a 42 kb segment located on the left arm of chromosome IV from Saccharomyces cerevisiae. Unpublished
2)	Ronnow, B. and Kielland-Brandt, M.C. (1992) Personal Communication, Mortimer Map Edition 11
3)	Wang HT, et al. (1994) Cloning, sequence, and disruption of the Saccharomyces diastaticus DAR1 gene encoding a glycerol-3-phosphate dehydrogenase. J Bacteriol 176(22):7091-5
4)	Hirayama T, et al. (1995) Cloning and characterization of seven cDNAs for hyperosmolarity-responsive (HOR) genes of Saccharomyces cerevisiae. Mol Gen Genet 249(2):127-38
5)	Nevoigt E and Stahl U (1997) Osmoregulation and glycerol metabolism in the yeast Saccharomyces cerevisiae. FEMS Microbiol Rev 21(3):231-41
6)	Nevoigt E and Stahl U (1996) Reduced pyruvate decarboxylase and increased glycerol-3-phosphate dehydrogenase [NAD+] levels enhance glycerol production in Saccharomyces cerevisiae. Yeast 12(13):1331-7
7)	Albertyn J, et al. (1994) GPD1, which encodes glycerol-3-phosphate dehydrogenase, is essential for growth under osmotic stress in Saccharomyces cerevisiae, and its expression is regulated by the high-osmolarity glycerol response pathway. Mol Cell Biol 14(6):4135-44
8)	Byrne KP and Wolfe KH (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
9)	Tkach JM, et al. (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
10)	Lee YJ, et al. (2012) Reciprocal phosphorylation of yeast glycerol-3-phosphate dehydrogenases in adaptation to distinct types of stress. Mol Cell Biol 32(22):4705-17