GPD1/YDL022W Summary Help

Standard Name GPD1 1, 2
Systematic Name YDL022W
Alias DAR1 3 , HOR1 4 , OSG1 5 , OSR5
Feature Type ORF, Verified
Description NAD-dependent glycerol-3-phosphate dehydrogenase; key enzyme of glycerol synthesis, essential for growth under osmotic stress; expression regulated by high-osmolarity glycerol response pathway; protein abundance increases in response to DNA replication stress; constitutively inactivated via phosphorylation by the protein kinases YPK1 and YPK2, dephosphorylation increases catalytic activity; GPD1 has a paralog, GPD2, that arose from the whole genome duplication (6, 7, 8, 9, 10)
Name Description Glycerol-3-Phosphate Dehydrogenase 7
Chromosomal Location
ChrIV:411825 to 413000 | ORF Map | GBrowse
Gene Ontology Annotations All GPD1 GO evidence and references
  View Computational GO annotations for GPD1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 19 genes
Classical genetics
Large-scale survey
72 total interaction(s) for 53 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 12
  • Affinity Capture-RNA: 5
  • Biochemical Activity: 1
  • Two-hybrid: 1

Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Rescue: 11
  • Negative Genetic: 23
  • Phenotypic Enhancement: 4
  • Phenotypic Suppression: 1
  • Positive Genetic: 2
  • Synthetic Growth Defect: 8
  • Synthetic Lethality: 2
  • Synthetic Rescue: 1

Expression Summary
Length (a.a.) 391
Molecular Weight (Da) 42,869
Isoelectric Point (pI) 5.26
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrIV:411825 to 413000 | ORF Map | GBrowse
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..1176 411825..413000 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002180
References cited on this page View Complete Literature Guide for GPD1
1) Andre, B., et al.,  (1995) The sequence of a 42 kb segment located on the left arm of chromosome IV from Saccharomyces cerevisiae. Unpublished
2) Ronnow, B. and Kielland-Brandt, M.C.  (1992) Personal Communication, Mortimer Map Edition 11
3) Wang HT, et al.  (1994) Cloning, sequence, and disruption of the Saccharomyces diastaticus DAR1 gene encoding a glycerol-3-phosphate dehydrogenase. J Bacteriol 176(22):7091-5
4) Hirayama T, et al.  (1995) Cloning and characterization of seven cDNAs for hyperosmolarity-responsive (HOR) genes of Saccharomyces cerevisiae. Mol Gen Genet 249(2):127-38
5) Nevoigt E and Stahl U  (1997) Osmoregulation and glycerol metabolism in the yeast Saccharomyces cerevisiae. FEMS Microbiol Rev 21(3):231-41
6) Nevoigt E and Stahl U  (1996) Reduced pyruvate decarboxylase and increased glycerol-3-phosphate dehydrogenase [NAD+] levels enhance glycerol production in Saccharomyces cerevisiae. Yeast 12(13):1331-7
7) Albertyn J, et al.  (1994) GPD1, which encodes glycerol-3-phosphate dehydrogenase, is essential for growth under osmotic stress in Saccharomyces cerevisiae, and its expression is regulated by the high-osmolarity glycerol response pathway. Mol Cell Biol 14(6):4135-44
8) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
9) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
10) Lee YJ, et al.  (2012) Reciprocal phosphorylation of yeast glycerol-3-phosphate dehydrogenases in adaptation to distinct types of stress. Mol Cell Biol 32(22):4705-17