NOP1/YDL014W Summary

NOP1 BASIC INFORMATION

Standard Name	NOP1
Systematic Name	YDL014W
Alias	LOT3
Feature Type	ORF, Verified
Description	Nucleolar protein, component of the small subunit processome complex, which is required for processing of pre-18S rRNA; has similarity to mammalian fibrillarin (1, 2, 3)
Name Description	NucleOlar Protein 2, 4

GO Annotations	All NOP1 GO evidence and references
	View Computational GO annotations for NOP1
Molecular Function
Manually curated	methyltransferase activity (IMP, IPI, ISS)
Biological Process
Manually curated	box C/D snoRNA 3'-end processing (IMP) RNA methylation (IMP, IPI, ISS) rRNA methylation (IMP)
Cellular Component
Manually curated	90S preribosome (IDA) box C/D snoRNP complex (IDA) nucleolus (IDA) small-subunit processome (IDA)
High-throughput	colocalizes_with ribosome (IDA)

Mutant Phenotype	All NOP1 Phenotype details and references
Classical genetics
null	inviable
repressible	35S pre-rRNA accumulation: increased aberrant 23S rRNA processing intermediate accumulation: increased 32S rRNA processing intermediate accumulation: absent 27SA rRNA processing intermediate accumulation: absent 20S rRNA processing intermediate accumulation: absent mature 18S rRNA accumulation: absent snR52 precursor accumulation: increased box C/D snoRNA construct derived from U14 (snR128) localization: abnormal box C/D family U14 snoRNA localization: abnormal
Large-scale survey
null	inviable

Interactions	NOP1 All interactions details and references
	354 total interaction(s) for 252 unique genes/features.
Physical Interactions	Affinity Capture-MS: 223 Affinity Capture-Western: 25 Biochemical Activity: 1 Co-localization: 5 Co-purification: 2 Reconstituted Complex: 1 Two-hybrid: 9
Genetic Interactions	Dosage Rescue: 2 Phenotypic Enhancement: 13 Phenotypic Suppression: 35 Synthetic Growth Defect: 1 Synthetic Lethality: 37

Sequence Information

ChrIV:427362 to 428345 | |

Genetic position: -1.93 cM

Last Update

Coordinates: 2008-06-05 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..984	427362..428345	2008-06-05	1996-07-31

External Links	All Associated Seq \| E.C. \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| UniProtKB

Primary SGDID	S000002172

NOP1 RESOURCES

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SGD ORF map	GBrowse

Click on histogram for expression summary
Expression Summary

ADDITIONAL INFORMATION for NOP1

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for NOP1]

1)	Tollervey D, et al. (1991) The small nucleolar RNP protein NOP1 (fibrillarin) is required for pre-rRNA processing in yeast. EMBO J 10(3):573-83
2)	Schimmang T, et al. (1989) A yeast nucleolar protein related to mammalian fibrillarin is associated with small nucleolar RNA and is essential for viability. EMBO J 8(13):4015-24
3)	Dragon F, et al. (2002) A large nucleolar U3 ribonucleoprotein required for 18S ribosomal RNA biogenesis. Nature 417(6892):967-70
4)	Henriquez R, et al. (1990) Isolation and sequencing of NOP1. A yeast gene encoding a nucleolar protein homologous to a human autoimmune antigen. J Biol Chem 265(4):2209-15

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