SLX5/YDL013W Summary Help

Standard Name SLX5 1
Systematic Name YDL013W
Alias HEX3 2 , ULS2 3
Feature Type ORF, Verified
Description Subunit of the Slx5-Slx8 SUMO-targeted ubiquitin ligase complex; stimulated by SUMO-modified substrates; contains a RING domain and two SIMs (SUMO-interacting motifs); forms SUMO-dependent nuclear foci, including DNA repair centers; the SUMO-targeted ubiquitin ligase complex is also known as the STUbL complex (1, 4, 5, 6, 7)
Name Description Synthetic Lethal of unknown [X] function 1
Chromosomal Location
ChrIV:429067 to 430926 | ORF Map | GBrowse
Genetic position: -1.69 cM
Gene Ontology Annotations All SLX5 GO evidence and references
  View Computational GO annotations for SLX5
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 1 genes
Classical genetics
reduction of function
Large-scale survey
657 total interaction(s) for 419 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 14
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 8
  • Biochemical Activity: 15
  • Co-purification: 1
  • Reconstituted Complex: 6
  • Two-hybrid: 39

Genetic Interactions
  • Dosage Lethality: 1
  • Dosage Rescue: 3
  • Negative Genetic: 275
  • Phenotypic Enhancement: 8
  • Phenotypic Suppression: 4
  • Positive Genetic: 85
  • Synthetic Growth Defect: 127
  • Synthetic Lethality: 57
  • Synthetic Rescue: 13

Expression Summary
Length (a.a.) 619
Molecular Weight (Da) 71,070
Isoelectric Point (pI) 6.66
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrIV:429067 to 430926 | ORF Map | GBrowse
Genetic position: -1.69 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..1860 429067..430926 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002171
References cited on this page View Complete Literature Guide for SLX5
1) Mullen JR, et al.  (2001) Requirement for three novel protein complexes in the absence of the Sgs1 DNA helicase in Saccharomyces cerevisiae. Genetics 157(1):103-18
2) Sternglanz, R.  (1993) Personal Communication, Mortimer Map Edition 12
3) Uzunova K, et al.  (2007) Ubiquitin-dependent Proteolytic Control of SUMO Conjugates. J Biol Chem 282(47):34167-75
4) Wang Z, et al.  (2006) Genetic Analysis Connects SLX5 and SLX8 to the SUMO Pathway in Saccharomyces cerevisiae. Genetics 172(3):1499-509
5) Xie Y, et al.  (2007) The Yeast Hex3{middle dot}Slx8 Heterodimer Is a Ubiquitin Ligase Stimulated by Substrate Sumoylation. J Biol Chem 282(47):34176-84
6) Ii T, et al.  (2007) The yeast Slx5-Slx8 DNA integrity complex displays ubiquitin ligase activity. Cell Cycle 6(22):2800-9
7) Cook CE, et al.  (2009) The SUMO-targeted ubiquitin ligase subunit Slx5 resides in nuclear foci and at sites of DNA breaks. Cell Cycle 8(7):1080-9