RPT2/YDL007W Summary Help

Standard Name RPT2 1
Systematic Name YDL007W
Alias YHS4 , YTA5 2
Feature Type ORF, Verified
Description ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; required for normal peptide hydrolysis by the core 20S particle; N-myristoylation of Rpt2p at Gly2 is involved in regulating the proper intracellular distribution of proteasome activity by controlling the nuclear localization of the 26S proteasome (3, 4, 5, 6)
Name Description Regulatory Particle Triple-A protein, or Regulatory Particle Triphosphatase 1
Chromosomal Location
ChrIV:438047 to 439360 | ORF Map | GBrowse
Gene Ontology Annotations All RPT2 GO evidence and references
  View Computational GO annotations for RPT2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 7 genes
Classical genetics
reduction of function
Large-scale survey
reduction of function
319 total interaction(s) for 194 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 107
  • Affinity Capture-RNA: 6
  • Affinity Capture-Western: 15
  • Biochemical Activity: 1
  • Co-fractionation: 1
  • Co-purification: 5
  • PCA: 4
  • Reconstituted Complex: 5
  • Two-hybrid: 2

Genetic Interactions
  • Dosage Growth Defect: 1
  • Negative Genetic: 126
  • Positive Genetic: 41
  • Synthetic Haploinsufficiency: 1
  • Synthetic Lethality: 4

Expression Summary
Length (a.a.) 437
Molecular Weight (Da) 48,828
Isoelectric Point (pI) 5.9
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrIV:438047 to 439360 | ORF Map | GBrowse
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..1314 438047..439360 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002165
References cited on this page View Complete Literature Guide for RPT2
1) Finley D, et al.  (1998) Unified nomenclature for subunits of the Saccharomyces cerevisiae proteasome regulatory particle. Trends Biochem Sci 23(7):244-5
2) Schnall R, et al.  (1994) Identification of a set of yeast genes coding for a novel family of putative ATPases with high similarity to constituents of the 26S protease complex. Yeast 10(9):1141-55
3) Glickman MH, et al.  (1998) The regulatory particle of the Saccharomyces cerevisiae proteasome. Mol Cell Biol 18(6):3149-62
4) Glickman MH, et al.  (1999) Functional analysis of the proteasome regulatory particle. Mol Biol Rep 26(1-2):21-8
5) Rubin DM, et al.  (1998) Active site mutants in the six regulatory particle ATPases reveal multiple roles for ATP in the proteasome. EMBO J 17(17):4909-19
6) Kimura A, et al.  (2012) N-myristoylation of the Rpt2 subunit regulates intracellular localization of the yeast 26S proteasome. Biochemistry 51(44):8856-66