MCD1/YDL003W Summary Help

Standard Name MCD1 1
Systematic Name YDL003W
Alias PDS3 , RHC21 2 , SCC1 3
Feature Type ORF, Verified
Description Essential alpha-kleisin subunit of the cohesin complex; required for sister chromatid cohesion in mitosis and meiosis; apoptosis induces cleavage and translocation of a C-terminal fragment to mitochondria; expression peaks in S phase (1, 4 and see Summary Paragraph)
Name Description Mitotic Chromosome Determinant 1
Chromosomal Location
ChrIV:444683 to 446383 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All MCD1 GO evidence and references
  View Computational GO annotations for MCD1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 4 genes
Resources
Classical genetics
conditional
null
overexpression
Large-scale survey
conditional
null
overexpression
reduction of function
unspecified
Resources
236 total interaction(s) for 119 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 37
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 35
  • Biochemical Activity: 8
  • Co-localization: 3
  • Co-purification: 3
  • FRET: 4
  • Reconstituted Complex: 4
  • Two-hybrid: 1

Genetic Interactions
  • Dosage Growth Defect: 2
  • Dosage Lethality: 3
  • Dosage Rescue: 8
  • Negative Genetic: 68
  • Phenotypic Enhancement: 4
  • Phenotypic Suppression: 12
  • Synthetic Growth Defect: 16
  • Synthetic Lethality: 23
  • Synthetic Rescue: 4

Resources
Expression Summary
histogram
Resources
Length (a.a.) 566
Molecular Weight (Da) 63,290
Isoelectric Point (pI) 4.35
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIV:444683 to 446383 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1701 444683..446383 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002161
SUMMARY PARAGRAPH for MCD1

MCD1 is an essential gene that encodes a subunit of the yeast cohesin complex, which is required for sister chromatid cohesion in mitosis and meiosis (3, 1, 2, 5, 6, 7, 8). Temperature-sensitive mutations in MCD1 cause defects in sister chromatid cohesion, chromosome condensation, and chromosome segregation (3, 1, 2, 5).

The yeast cohesin complex includes Smc1p and Smc3p, two members of a conserved family of chromosomal ATPases, and Irr1p (1, 5, 7, 8). Cohesin associates with centromeres and other discrete sites along chromosome arms prior to metaphase; the association with centromeres requires the centromere protein Mif2p, the centromere binding complex CBF3, and Cse4p (9, 10, 11). Scc2p is not a stoichiometric cohesin subunit, but is required for the cohesin complex to associate with chromosomes (5).

The level of Mcd1p is cell cycle regulated, and peaks in S phase (3, 1); Mcd1p must be present during S phase for sister chromatid cohesion to be established (12). Mcd1p dissociates from chromosomes upon the loss of sister chromatid cohesion at the metaphase-to-anaphase transition (3). Dissociation of Mcd1p from chromatin requires Esp1, a separin that stimulates proteolysis of Mcd1p (13, 14).

Mcd1p shares homology with the S. pombe Rad21 protein and with putative proteins encoded by ORFs from human, mouse, and C. elegans (1). Cohesin complexes have been identified in other eukaryotes, including Xenopus, C. elegans, S. pombe, and human (7).

Last updated: 2000-05-04 Contact SGD

References cited on this page View Complete Literature Guide for MCD1
1) Guacci V, et al.  (1997) A direct link between sister chromatid cohesion and chromosome condensation revealed through the analysis of MCD1 in S. cerevisiae. Cell 91(1):47-57
2) Heo SJ, et al.  (1998) The RHC21 gene of budding yeast, a homologue of the fission yeast rad21+ gene, is essential for chromosome segregation. Mol Gen Genet 257(2):149-56
3) Michaelis C, et al.  (1997) Cohesins: chromosomal proteins that prevent premature separation of sister chromatids. Cell 91(1):35-45
4) Yang H, et al.  (2008) Cleavage of Mcd1 by Caspase-like Protease Esp1 Promotes Apoptosis in Budding Yeast. Mol Biol Cell 19(5):2127-34
5) Toth A, et al.  (1999) Yeast cohesin complex requires a conserved protein, Eco1p(Ctf7), to establish cohesion between sister chromatids during DNA replication. Genes Dev 13(3):320-33
6) Klein F, et al.  (1999) A central role for cohesins in sister chromatid cohesion, formation of axial elements, and recombination during yeast meiosis. Cell 98(1):91-103
7) Hirano T  (1999) SMC-mediated chromosome mechanics: a conserved scheme from bacteria to vertebrates? Genes Dev 13(1):11-9
8) Strunnikov AV and Jessberger R  (1999) Structural maintenance of chromosomes (SMC) proteins: conserved molecular properties for multiple biological functions. Eur J Biochem 263(1):6-13
9) Tanaka T, et al.  (1999) Identification of cohesin association sites at centromeres and along chromosome arms. Cell 98(6):847-58
10) Blat Y and Kleckner N  (1999) Cohesins bind to preferential sites along yeast chromosome III, with differential regulation along arms versus the centric region. Cell 98(2):249-59
11) Megee PC, et al.  (1999) The centromeric sister chromatid cohesion site directs Mcd1p binding to adjacent sequences. Mol Cell 4(3):445-50
12) Uhlmann F and Nasmyth K  (1998) Cohesion between sister chromatids must be established during DNA replication. Curr Biol 8(20):1095-101
13) Ciosk R, et al.  (1998) An ESP1/PDS1 complex regulates loss of sister chromatid cohesion at the metaphase to anaphase transition in yeast. Cell 93(6):1067-76
14) Uhlmann F, et al.  (1999) Sister-chromatid separation at anaphase onset is promoted by cleavage of the cohesin subunit Scc1. Nature 400(6739):37-42