Standard Name	RPN12 1
Systematic Name	YFR052W
Alias	NIN1 2
Feature Type	ORF, Verified
Description	Subunit of the 19S regulatory particle of the 26S proteasome lid; synthetically lethal with RPT1, which is an ATPase component of the 19S regulatory particle; physically interacts with Nob1p and Rpn3p; protein abundance increases in response to DNA replication stress (3, 4, 5)
Name Description	Regulatory Particle Non-ATPase 1

Chromosomal Location	ChrVI:252505 to 253329 | ORF Map | GBrowse

	View Computational GO annotations for RPN12
Molecular Function
Manually curated	molecular_function unknown (ND)
Biological Process
Manually curated	ubiquitin-dependent protein catabolic process (IMP)
Cellular Component
Manually curated	proteasome regulatory particle, lid subcomplex (IDA) proteasome storage granule (IDA)

Classical genetics
conditional	UV resistance: decreased cell cycle progression in G2 phase: arrested cell cycle progression through the G1/S phase transition: abnormal cell cycle progression through the G2/M phase transition: abnormal chromosome segregation: abnormal heat sensitivity: increased mitotic recombination: increased rate nuclear morphology: abnormal resistance to canavanine: decreased resistance to cadmium(2+): decreased sporulation: absent
null	inviable
Large-scale survey
null	cell size: increased filamentous growth: increased haploinsufficient inviable resistance to clioquinol: decreased
reduction of function	chromosome/plasmid maintenance: abnormal competitive fitness: increased telomere length: decreased
Resources	PROPHECY | PhenoM | SCMD | ScreenTroll | Yeast Fitness Database

	348 total interaction(s) for 174 unique genes/features.
Physical Interactions	Affinity Capture-MS: 135 Affinity Capture-RNA: 1 Affinity Capture-Western: 37 Co-fractionation: 1 Co-purification: 5 Reconstituted Complex: 11 Two-hybrid: 8
Genetic Interactions	Dosage Growth Defect: 2 Dosage Lethality: 1 Dosage Rescue: 3 Negative Genetic: 113 Positive Genetic: 15 Synthetic Growth Defect: 3 Synthetic Haploinsufficiency: 1 Synthetic Lethality: 12
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | AspGD Homologs | CGD Homologs | CandidaDB Homologs | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrVI:252505 to 253329 | ORF Map | GBrowse
Last Update	Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..825 252505..253329 2011-02-03 1996-07-31
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI1631 S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CBS7960 S.c. Strain CEN.PK113-7D S.c. Strain CLIB215 S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain M22 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain T73 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain Y10 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000001948