RPN12/YFR052W Summary Help

Standard Name RPN12 1
Systematic Name YFR052W
Alias NIN1 2
Feature Type ORF, Verified
Description Subunit of the 19S regulatory particle of the 26S proteasome lid; synthetically lethal with RPT1, which is an ATPase component of the 19S regulatory particle; physically interacts with Nob1p and Rpn3p; protein abundance increases in response to DNA replication stress (3, 4, 5)
Name Description Regulatory Particle Non-ATPase 1
Chromosomal Location
ChrVI:252505 to 253329 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All RPN12 GO evidence and references
  View Computational GO annotations for RPN12
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 6 genes
Resources
Classical genetics
conditional
null
Large-scale survey
null
reduction of function
Resources
366 total interaction(s) for 189 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 138
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 37
  • Co-fractionation: 2
  • Co-purification: 5
  • Reconstituted Complex: 11
  • Two-hybrid: 8

Genetic Interactions
  • Dosage Growth Defect: 2
  • Dosage Lethality: 1
  • Dosage Rescue: 3
  • Negative Genetic: 122
  • Positive Genetic: 19
  • Synthetic Growth Defect: 3
  • Synthetic Haploinsufficiency: 1
  • Synthetic Lethality: 12

Resources
Expression Summary
histogram
Resources
Length (a.a.) 274
Molecular Weight (Da) 31,919
Isoelectric Point (pI) 4.64
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrVI:252505 to 253329 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..825 252505..253329 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001948
References cited on this page View Complete Literature Guide for RPN12
1) Finley D, et al.  (1998) Unified nomenclature for subunits of the Saccharomyces cerevisiae proteasome regulatory particle. Trends Biochem Sci 23(7):244-5
2) Nisogi H, et al.  (1992) A new essential gene of Saccharomyces cerevisiae, a defect in it may result in instability of nucleus. Exp Cell Res 200(1):48-57
3) Takeuchi J and Toh-e A  (1999) Genetic evidence for interaction between components of the yeast 26S proteasome: combination of a mutation in RPN12 (a lid component gene) with mutations in RPT1 (an ATPase gene) causes synthetic lethality. Mol Gen Genet 262(1):145-53
4) Tone Y, et al.  (2000) Nob1p, a new essential protein, associates with the 26S proteasome of growing saccharomyces cerevisiae cells. Gene 243(1-2):37-45
5) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76