PRE4/YFR050C Summary

PRE4 BASIC INFORMATION

Standard Name	PRE4 1
Systematic Name	YFR050C
Feature Type	ORF, Verified
Description	Beta 7 subunit of the 20S proteasome (2, 3)
Name Description	PRoteinase yscE 1

GO Annotations	All PRE4 GO evidence and references
	View Computational GO annotations for PRE4
Molecular Function
Manually curated	endopeptidase activity (TAS)
Biological Process
Manually curated	proteasome assembly (IGI) ubiquitin-dependent protein catabolic process (TAS)
Cellular Component
Manually curated	proteasome core complex, beta-subunit complex (IPI, TAS) proteasome storage granule (IDA)

Mutant Phenotype	All PRE4 Phenotype details and references
Large-scale survey
conditional	heat sensitivity: increased
null	inviable

Interactions	PRE4 All interactions details and references
	117 total interaction(s) for 40 unique genes/features.
Physical Interactions	Affinity Capture-MS: 41 Affinity Capture-Western: 6 Co-crystal Structure: 5 Co-fractionation: 1 Co-purification: 1 Two-hybrid: 7
Genetic Interactions	Dosage Lethality: 4 Dosage Rescue: 1 Phenotypic Enhancement: 3 Synthetic Growth Defect: 25 Synthetic Rescue: 23

Sequence Information

ChrVI:249853 to 249053 | |

Note: this feature is encoded on the Crick strand.

Last Update

Coordinates: 2003-09-26 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..801	249853..249053	2003-09-26	1996-07-31

External Links	All Associated Seq \| E.C. \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| UniProtKB

Primary SGDID	S000001946

PRE4 RESOURCES

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SGD ORF map	GBrowse

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Expression Summary

ADDITIONAL INFORMATION for PRE4

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for PRE4]

1)	Hilt W, et al. (1993) The PRE4 gene codes for a subunit of the yeast proteasome necessary for peptidylglutamyl-peptide-hydrolyzing activity. Mutations link the proteasome to stress- and ubiquitin-dependent proteolysis. J Biol Chem 268(5):3479-86
2)	Groll M, et al. (1999) The catalytic sites of 20S proteasomes and their role in subunit maturation: a mutational and crystallographic study. Proc Natl Acad Sci U S A 96(20):10976-83
3)	Jager S, et al. (1999) Proteasome beta-type subunits: unequal roles of propeptides in core particle maturation and a hierarchy of active site function. J Mol Biol 291(4):997-1013

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