CDC26/YFR036W Summary Help

Standard Name CDC26 1
Systematic Name YFR036W
Alias HIT3 , SCD26
Feature Type ORF, Verified
Description Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; relocalizes to the cytosol in response to hypoxia (2, 3 and see Summary Paragraph)
Name Description Cell Division Cycle 4
Chromosomal Location
ChrVI:226963 to 227337 | ORF Map | GBrowse
Gbrowse
Genetic position: 52 cM
Gene Ontology Annotations All CDC26 GO evidence and references
  View Computational GO annotations for CDC26
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 3 genes
Resources
Classical genetics
conditional
null
Large-scale survey
null
Resources
123 total interaction(s) for 80 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 19
  • Affinity Capture-Western: 12
  • Biochemical Activity: 2
  • Protein-RNA: 1
  • Two-hybrid: 2

Genetic Interactions
  • Dosage Rescue: 1
  • Negative Genetic: 41
  • Phenotypic Enhancement: 1
  • Positive Genetic: 4
  • Synthetic Growth Defect: 14
  • Synthetic Lethality: 25
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 124
Molecular Weight (Da) 14,081
Isoelectric Point (pI) 4.33
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrVI:226963 to 227337 | ORF Map | GBrowse
SGD ORF map
Genetic position: 52 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..375 226963..227337 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001932
SUMMARY PARAGRAPH for CDC26

CDC26 is a subunit of the anaphase-promoting complex (APC), an E3 ubiquitin ligase in the ubiquitin-mediated proteolysis pathway (2). The APC ubiquitin ligase helps regulate the metaphase/anaphase transition and exit from mitosis/G1 entry through ubiquitination of various substrates. These include mitotic cyclins, the sister chromatid separation inhibitor Pds1p, the Kip1p and Cin8p motor proteins, Cdc5p, and the spindle disassemblyfactor, Ase1p (5, 6, 7, 8, 9, 10).

Cdc26p, although a nonessential APC subunit, seems to be important for assembly of the complete APC particle. In immunoprecipitates from cdc26 strains, the amounts of Cdc16p, Cdc27p, and Apc9p are reduced, whereas the other APC subunits are still associated with each other (11). Thus, Cdc26p appears to stabilize the interaction of three core subunits (Cdc16p, Cdc27p, Apc9p) with the rest of the APC complex (12)(11).

Cdc26p is also a heat shock protein whose function is only required for growth at high temperatures (13)(12). At 37 degrees celsius, cdc26 mutants arrest as large, budded cells containing a short mitotic spindle in an undivided nucleus positioned at the bud neck (12). In addition, G1-arrested cdc26 mutants are also defective in ubiquitination of the mitotic cyclins Clb2p and Clb3p, two substrates of the APC (12). CDC26 orthologs have been identified in various species including worms, mice, rats, and humans (14).

Last updated: 2005-05-04 Contact SGD

References cited on this page View Complete Literature Guide for CDC26
1) Hartwell LH, et al.  (1973) Genetic Control of the Cell Division Cycle in Yeast: V. Genetic Analysis of cdc Mutants. Genetics 74(2):267-286
2) Zachariae W and Nasmyth K  (1999) Whose end is destruction: cell division and the anaphase-promoting complex. Genes Dev 13(16):2039-58
3) Ghosh Dastidar R, et al.  (2012) The nuclear localization of SWI/SNF proteins is subjected to oxygen regulation. Cell Biosci 2(1):30
4) Hartwell LH, et al.  (1970) Genetic control of the cell-division cycle in yeast. I. Detection of mutants. Proc Natl Acad Sci U S A 66(2):352-9
5) Juang YL, et al.  (1997) APC-mediated proteolysis of Ase1 and the morphogenesis of the mitotic spindle. Science 275(5304):1311-4
6) Cohen-Fix O, et al.  (1996) Anaphase initiation in Saccharomyces cerevisiae is controlled by the APC-dependent degradation of the anaphase inhibitor Pds1p. Genes Dev 10(24):3081-93
7) Shirayama M, et al.  (1998) The Polo-like kinase Cdc5p and the WD-repeat protein Cdc20p/fizzy are regulators and substrates of the anaphase promoting complex in Saccharomyces cerevisiae. EMBO J 17(5):1336-49
8) Gordon DM and Roof DM  (2001) Degradation of the kinesin Kip1p at anaphase onset is mediated by the anaphase-promoting complex and Cdc20p. Proc Natl Acad Sci U S A 98(22):12515-20
9) Hildebrandt ER and Hoyt MA  (2001) Cell cycle-dependent degradation of the Saccharomyces cerevisiae spindle motor Cin8p requires APC(Cdh1) and a bipartite destruction sequence. Mol Biol Cell 12(11):3402-16
10) Zachariae W and Nasmyth K  (1996) TPR proteins required for anaphase progression mediate ubiquitination of mitotic B-type cyclins in yeast. Mol Biol Cell 7(5):791-801
11) Zachariae W, et al.  (1998) Mass spectrometric analysis of the anaphase-promoting complex from yeast: identification of a subunit related to cullins. Science 279(5354):1216-9
12) Zachariae W, et al.  (1996) Identification of subunits of the anaphase-promoting complex of Saccharomyces cerevisiae. Science 274(5290):1201-4
13) Araki H, et al.  (1992) The CDC26 gene of Saccharomyces cerevisiae is required for cell growth only at high temperature. Mol Gen Genet 231(2):329-31
14) Gmachl M, et al.  (2000) The RING-H2 finger protein APC11 and the E2 enzyme UBC4 are sufficient to ubiquitinate substrates of the anaphase-promoting complex. Proc Natl Acad Sci U S A 97(16):8973-8