SMC2/YFR031C Summary Help

SMC2 BASIC INFORMATION

Standard Name SMC2
Systematic Name YFR031C
Feature Type ORF, Verified
Description Subunit of the condensin complex; essential SMC chromosomal ATPase family member that forms a complex with Smc4p to form the active ATPase; Smc2p/Smc4p complex binds DNA; required for clustering of tRNA genes at the nucleolus (1, 2, 3 and see Summary Paragraph)
Name Description Structural Maintenance of Chromosomes 1
GO Annotations All SMC2 GO evidence and references
    View Computational GO annotations for SMC2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Mutant Phenotype All SMC2 Phenotype details and references
Classical genetics
conditional
Large-scale survey
null
Interactions SMC2 All interactions details and references
41 total interaction(s) for 27 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 17
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 7
  • Co-purification: 1
  • Reconstituted Complex: 1
  • Two-hybrid: 8
Genetic Interactions
  • Dosage Rescue: 2
  • Phenotypic Enhancement: 1
  • Synthetic Growth Defect: 3
Sequence Information
ChrVI:220094 to 216582 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Last Update Coordinates: 2003-09-26 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..3513 220094..216582 2003-09-26 1996-07-31
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | UniProtKB
Primary SGDIDS000001927

SMC2 RESOURCES

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SGD ORF mapGBrowse
SGD ORF map
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  • Sequence Analysis Tools
  • Protein Info & Structure
  • Localization Resources
  • Interactions
  • Phenotype Resources
  • Maps & Displays
  • Comparison Resources
  • Functional Analysis

Click on histogram for expression summary
Expression Summary histogram

ADDITIONAL INFORMATION for SMC2

SUMMARY PARAGRAPH for SMC2

SMC2 is an essential gene that encodes a member of a ubiquitous family of chromosome-associated ATPases (1, 4, 5, 6). SMC proteins are found in eukaryotes, prokaryotes, and archaea, and appear to play roles in chromosome dynamics (5, 6). In eukaryotes, SMC proteins form two kinds of heterodimers, corresponding to Smc1p-Smc3p and Smc2p-Smc4p in yeast (5, 6). The Smc2p-Smc4p heterodimer interacts with additional proteins, Brn1p, Ycg1p, and Loc7p, to form the yeast condensin complex (6, 5, 7, 8). Originally identified in Xenopus egg extracts, the condensin complex is required for chromosome condensation (9, 6, 5). Condensin complexes have been identified in many eukaryotes, including Drosophila, human, and S. pombe (5). The temperature-sensitive mutation smc2-6 causes a defect in chromosome segregation, and causes partial chromosome decondensation in cells arrested in mitosis (1). The smc2-6 phenotype can be suppressed by overexpression of TID3, which encodes a component of the spindle pole body; Tid3p also interacts physically with Smc2p (10).

Last updated: 2000-05-04

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for SMC2]

1) Strunnikov AV, et al.  (1995) SMC2, a Saccharomyces cerevisiae gene essential for chromosome segregation and condensation, defines a subgroup within the SMC family. Genes Dev 9(5):587-99
2) Stray JE and Lindsley JE  (2003) Biochemical analysis of the yeast condensin Smc2/4 complex: an ATPase that promotes knotting of circular DNA. J Biol Chem 278(28):26238-48
3) Haeusler RA, et al.  (2008) Clustering of yeast tRNA genes is mediated by specific association of condensin with tRNA gene transcription complexes. Genes Dev 22(16):2204-14
4) Akhmedov AT, et al.  (1998) Structural maintenance of chromosomes protein C-terminal domains bind preferentially to DNA with secondary structure. J Biol Chem 273(37):24088-94
5) Hirano T  (1999) SMC-mediated chromosome mechanics: a conserved scheme from bacteria to vertebrates? Genes Dev 13(1):11-9
6) Strunnikov AV and Jessberger R  (1999) Structural maintenance of chromosomes (SMC) proteins: conserved molecular properties for multiple biological functions. Eur J Biochem 263(1):6-13
7) Ouspenski II, et al.  (2000) Chromosome condensation factor Brn1p is required for chromatid separation in mitosis. Mol Biol Cell 11(4):1305-13
8) Lavoie BD, et al.  (2000) Mitotic chromosome condensation requires Brn1p, the yeast homologue of Barren. Mol Biol Cell 11(4):1293-304
9) Hirano T, et al.  (1997) Condensins, chromosome condensation protein complexes containing XCAP-C, XCAP-E and a Xenopus homolog of the Drosophila Barren protein. Cell 89(4):511-21
10) Zheng L, et al.  (1999) Hec1p, an evolutionarily conserved coiled-coil protein, modulates chromosome segregation through interaction with SMC proteins. Mol Cell Biol 19(8):5417-28