SMC2/YFR031C Summary Help

Standard Name SMC2 1
Systematic Name YFR031C
Feature Type ORF, Verified
Description Subunit of the condensin complex; condensin reorganizes chromosomes during both mitosis and meiosis; essential SMC chromosomal ATPase family member that forms a subcomplex with Smc2p that has ATP-hydrolyzing and DNA-binding activity, but other condensin subunits are required for chromatin binding; required for clustering of tRNA genes at the nucleolus (1, 2, 3, 4, 5 and see Summary Paragraph)
Name Description Structural Maintenance of Chromosomes 1
Chromosomal Location
ChrVI:220106 to 216594 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All SMC2 GO evidence and references
  View Computational GO annotations for SMC2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 1 genes
Classical genetics
Large-scale survey
reduction of function
79 total interaction(s) for 54 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 24
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 10
  • Co-purification: 1
  • Reconstituted Complex: 1
  • Two-hybrid: 8

Genetic Interactions
  • Dosage Rescue: 5
  • Phenotypic Enhancement: 3
  • Synthetic Growth Defect: 4
  • Synthetic Lethality: 22

Expression Summary
Length (a.a.) 1,170
Molecular Weight (Da) 133,927
Isoelectric Point (pI) 7.21
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrVI:220106 to 216594 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 2011-02-03 | Sequence: 2011-02-03
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..3513 220106..216594 2011-02-03 2011-02-03
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001927

SMC2 is an essential gene that encodes a member of a ubiquitous family of chromosome-associated ATPases (1, 6, 7, 8). SMC proteins are found in eukaryotes, prokaryotes, and archaea, and appear to play roles in chromosome dynamics (7, 8). In eukaryotes, SMC proteins form two kinds of heterodimers, corresponding to Smc1p-Smc3p and Smc2p-Smc4p in yeast (7, 8). The Smc2p-Smc4p heterodimer interacts with additional proteins, Brn1p, Ycg1p, and Loc7p, to form the yeast condensin complex (8, 7, 9, 10). Originally identified in Xenopus egg extracts, the condensin complex is required for chromosome condensation (11, 8, 7). Condensin complexes have been identified in many eukaryotes, including Drosophila, human, and S. pombe (7). The temperature-sensitive mutation smc2-6 causes a defect in chromosome segregation, and causes partial chromosome decondensation in cells arrested in mitosis (1). The smc2-6 phenotype can be suppressed by overexpression of TID3, which encodes a component of the spindle pole body; Tid3p also interacts physically with Smc2p (12).

Last updated: 2000-05-04 Contact SGD

References cited on this page View Complete Literature Guide for SMC2
1) Strunnikov AV, et al.  (1995) SMC2, a Saccharomyces cerevisiae gene essential for chromosome segregation and condensation, defines a subgroup within the SMC family. Genes Dev 9(5):587-99
2) Freeman L, et al.  (2000) The condensin complex governs chromosome condensation and mitotic transmission of rDNA. J Cell Biol 149(4):811-24
3) Stray JE and Lindsley JE  (2003) Biochemical analysis of the yeast condensin Smc2/4 complex: an ATPase that promotes knotting of circular DNA. J Biol Chem 278(28):26238-48
4) Yu HG and Koshland DE  (2003) Meiotic condensin is required for proper chromosome compaction, SC assembly, and resolution of recombination-dependent chromosome linkages. J Cell Biol 163(5):937-47
5) Haeusler RA, et al.  (2008) Clustering of yeast tRNA genes is mediated by specific association of condensin with tRNA gene transcription complexes. Genes Dev 22(16):2204-14
6) Akhmedov AT, et al.  (1998) Structural maintenance of chromosomes protein C-terminal domains bind preferentially to DNA with secondary structure. J Biol Chem 273(37):24088-94
7) Hirano T  (1999) SMC-mediated chromosome mechanics: a conserved scheme from bacteria to vertebrates? Genes Dev 13(1):11-9
8) Strunnikov AV and Jessberger R  (1999) Structural maintenance of chromosomes (SMC) proteins: conserved molecular properties for multiple biological functions. Eur J Biochem 263(1):6-13
9) Ouspenski II, et al.  (2000) Chromosome condensation factor Brn1p is required for chromatid separation in mitosis. Mol Biol Cell 11(4):1305-13
10) Lavoie BD, et al.  (2000) Mitotic chromosome condensation requires Brn1p, the yeast homologue of Barren. Mol Biol Cell 11(4):1293-304
11) Hirano T, et al.  (1997) Condensins, chromosome condensation protein complexes containing XCAP-C, XCAP-E and a Xenopus homolog of the Drosophila Barren protein. Cell 89(4):511-21
12) Zheng L, et al.  (1999) Hec1p, an evolutionarily conserved coiled-coil protein, modulates chromosome segregation through interaction with SMC proteins. Mol Cell Biol 19(8):5417-28