ECO1/YFR027W Summary Help

Standard Name ECO1 1
Systematic Name YFR027W
Alias CTF7 2
Feature Type ORF, Verified
Description Acetyltransferase; required for establishment of sister chromatid cohesion; acetylates Mps3p to regulate nuclear organization; modifies Smc3p at replication forks and Mcd1p in response to dsDNA breaks; phosphorylated by three kinases (Cdc28p, Cdc7p, Mck1p) to generate pair of phosphates spaced precisely for recognition by ubiquitin ligase SCF-Cdc4; mutations in human homolog ESCO2 cause Roberts syndrome; relative distribution to nucleus increases upon DNA replication stress (1, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15)
Name Description Establishment of COhesion 1
Chromosomal Location
ChrVI:207452 to 208297 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All ECO1 GO evidence and references
  View Computational GO annotations for ECO1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 1 genes
Resources
Classical genetics
conditional
repressible
unspecified
Large-scale survey
conditional
null
reduction of function
Resources
95 total interaction(s) for 42 unique genes/features.
Physical Interactions
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 15
  • Biochemical Activity: 20
  • Protein-peptide: 1
  • Reconstituted Complex: 3
  • Two-hybrid: 2

Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Rescue: 4
  • Negative Genetic: 1
  • Phenotypic Enhancement: 2
  • Phenotypic Suppression: 4
  • Synthetic Growth Defect: 9
  • Synthetic Lethality: 17
  • Synthetic Rescue: 15

Resources
Expression Summary
histogram
Resources
Length (a.a.) 281
Molecular Weight (Da) 31,845
Isoelectric Point (pI) 10.75
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrVI:207452 to 208297 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..846 207452..208297 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001923
References cited on this page View Complete Literature Guide for ECO1
1) Toth A, et al.  (1999) Yeast cohesin complex requires a conserved protein, Eco1p(Ctf7), to establish cohesion between sister chromatids during DNA replication. Genes Dev 13(3):320-33
2) Spencer F, et al.  (1990) Mitotic chromosome transmission fidelity mutants in Saccharomyces cerevisiae. Genetics 124(2):237-49
3) Skibbens RV, et al.  (1999) Ctf7p is essential for sister chromatid cohesion and links mitotic chromosome structure to the DNA replication machinery. Genes Dev 13(3):307-19
4) Sjogren C and Nasmyth K  (2001) Sister chromatid cohesion is required for postreplicative double-strand break repair in Saccharomyces cerevisiae. Curr Biol 11(12):991-5
5) Vega H, et al.  (2005) Roberts syndrome is caused by mutations in ESCO2, a human homolog of yeast ECO1 that is essential for the establishment of sister chromatid cohesion. Nat Genet 37(5):468-70
6) Lengronne A, et al.  (2006) Establishment of sister chromatid cohesion at the S. cerevisiae replication fork. Mol Cell 23(6):787-99
7) Unal E, et al.  (2007) DNA double-strand breaks trigger genome-wide sister-chromatid cohesion through Eco1 (Ctf7). Science 317(5835):245-8
8) Zhang J, et al.  (2008) Acetylation of Smc3 by Eco1 is required for S phase sister chromatid cohesion in both human and yeast. Mol Cell 31(1):143-51
9) Unal E, et al.  (2008) A molecular determinant for the establishment of sister chromatid cohesion. Science 321(5888):566-9
10) Ben-Shahar TR, et al.  (2008) Eco1-dependent cohesin acetylation during establishment of sister chromatid cohesion. Science 321(5888):563-6
11) Heidinger-Pauli JM, et al.  (2009) Distinct targets of the Eco1 acetyltransferase modulate cohesion in S phase and in response to DNA damage. Mol Cell 34(3):311-21
12) Onn I, et al.  (2009) The zinc finger of Eco1 enhances its acetyltransferase activity during sister chromatid cohesion. Nucleic Acids Res 37(18):6126-34
13) Ghosh S, et al.  (2012) Acetylation of the SUN protein Mps3 by Eco1 regulates its function in nuclear organization. Mol Biol Cell 23(13):2546-59
14) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
15) Lyons NA, et al.  (2013) Sequential primed kinases create a damage-responsive phosphodegron on Eco1. Nat Struct Mol Biol 20(2):194-201