HIS2/YFR025C Summary Help

Standard Name HIS2 1
Systematic Name YFR025C
Feature Type ORF, Verified
Description Histidinolphosphatase; catalyzes the eighth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control (2, 3, 4 and see Summary Paragraph)
Name Description HIStidine requiring 1
Chromosomal Location
ChrVI:204750 to 203743 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Genetic position: 32 cM
Gene Ontology Annotations All HIS2 GO evidence and references
  View Computational GO annotations for HIS2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
High-throughput
Regulators 4 genes
Resources
Pathways
Classical genetics
unspecified
Large-scale survey
null
Resources
7 total interaction(s) for 6 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 2
  • Affinity Capture-RNA: 2
  • Co-purification: 1
  • PCA: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 335
Molecular Weight (Da) 38,582
Isoelectric Point (pI) 5.83
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrVI:204750 to 203743 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Genetic position: 32 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1008 204750..203743 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001921
SUMMARY PARAGRAPH for HIS2

HIS2 encodes histidinol-phosphatase, which catalyzes the eighth step in histidine biosynthesis (3). The biosynthesis of histidine has been most extensively studied in Salmonella typhimurium and E. coli. The reactions and enzymes involved in histidine biosynthesis have been identified in many organisms, and are thoroughly reviewed in Alifano et al. (3). Mutations in HIS2, as well as in genes encoding other histidine biosynthetic enzymes, cause histidine auxotrophy and sensitivity to copper, cobalt, and nickel salts (2). Transcription of HIS2 is regulated by general amino acid control, in which the transcription factor Gcn4p plays a key role (reviewed in 5). There is a recombination hotspot near the HIS2 coding sequence (6, 7).

Last updated: 1999-11-10 Contact SGD

References cited on this page View Complete Literature Guide for HIS2
1) Gorman JA and Gorman J  (1971) Genetic analysis of a gene required for the expression of allele-specific missense suppression in Saccharomyces cerevisiae. Genetics 67(3):337-52
2) Pearce DA and Sherman F  (1999) Toxicity of copper, cobalt, and nickel salts is dependent on histidine metabolism in the yeast Saccharomyces cerevisiae. J Bacteriol 181(16):4774-9
3) Alifano P, et al.  (1996) Histidine biosynthetic pathway and genes: structure, regulation, and evolution. Microbiol Rev 60(1):44-69
4) Fink GR  (1964) GENE-ENZYME RELATIONS IN HISTIDINE BIOSYNTHESIS IN YEAST. Science 146(3643):525-7
5) Hinnebusch A  (1992) "General and Pathway-specific Regulatory Mechanisms Controlling the Synthesis of Amino Acid Biosynthetic Enzymes in Saccharomyces cerevisiae". Pp. 319-414 in The Molecular and Cellular Biology of the Yeast Saccharomyces: Gene Expression, edited by Jones EW, Pringle JR and Broach JR. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press
6) Malone RE, et al.  (1994) Analysis of a recombination hotspot for gene conversion occurring at the HIS2 gene of Saccharomyces cerevisiae. Genetics 137(1):5-18
7) Bullard SA, et al.  (1996) Double strand breaks at the HIS2 recombination hot spot in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A 93(23):13054-9