UBP6/YFR010W Summary Help

Standard Name UBP6 1
Systematic Name YFR010W
Feature Type ORF, Verified
Description Ubiquitin-specific protease; situated in the base subcomplex of the 26S proteasome, releases free ubiquitin from branched polyubiquitin chains; negatively regulates degradation of ubiquitinated proteins by the proteasome; works in opposition to Hul5p polyubiquitin elongation activity; mutant has aneuploidy tolerance (1, 2, 3, 4, 5, 6, 7)
Name Description UBiquitin-specific Protease 1
Chromosomal Location
ChrVI:165067 to 166566 | ORF Map | GBrowse
Gene Ontology Annotations All UBP6 GO evidence and references
  View Computational GO annotations for UBP6
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Classical genetics
Large-scale survey
638 total interaction(s) for 358 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 113
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 6
  • Biochemical Activity: 8
  • Co-purification: 5
  • PCA: 4
  • Reconstituted Complex: 5
  • Two-hybrid: 1

Genetic Interactions
  • Dosage Growth Defect: 1
  • Negative Genetic: 360
  • Phenotypic Enhancement: 9
  • Phenotypic Suppression: 3
  • Positive Genetic: 39
  • Synthetic Growth Defect: 38
  • Synthetic Lethality: 25
  • Synthetic Rescue: 19

Expression Summary
Length (a.a.) 499
Molecular Weight (Da) 57,110
Isoelectric Point (pI) 7.14
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrVI:165067 to 166566 | ORF Map | GBrowse
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..1500 165067..166566 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001906
References cited on this page View Complete Literature Guide for UBP6
1) Park KC, et al.  (1997) Purification and characterization of UBP6, a new ubiquitin-specific protease in Saccharomyces cerevisiae. Arch Biochem Biophys 347(1):78-84
2) Hanna J, et al.  (2003) Ubiquitin depletion as a key mediator of toxicity by translational inhibitors. Mol Cell Biol 23(24):9251-61
3) Chernova TA, et al.  (2003) Pleiotropic effects of Ubp6 loss on drug sensitivities and yeast prion are due to depletion of the free ubiquitin pool. J Biol Chem 278(52):52102-15
4) Guterman A and Glickman MH  (2004) Complementary roles for Rpn11 and Ubp6 in deubiquitination and proteolysis by the proteasome. J Biol Chem 279(3):1729-38
5) Hanna J, et al.  (2006) Deubiquitinating enzyme Ubp6 functions noncatalytically to delay proteasomal degradation. Cell 127(1):99-111
6) Crosas B, et al.  (2006) Ubiquitin chains are remodeled at the proteasome by opposing ubiquitin ligase and deubiquitinating activities. Cell 127(7):1401-13
7) Torres EM, et al.  (2010) Identification of aneuploidy-tolerating mutations. Cell 143(1):71-83