RPN11/YFR004W Summary Help

Standard Name RPN11 1
Systematic Name YFR004W
Alias MPR1
Feature Type ORF, Verified
Description Metalloprotease subunit of 19S regulatory particle; part of 26S proteasome lid; couples the deubiquitination and degradation of proteasome substrates; involved, independent of catalytic activity, in fission of mitochondria and peroxisomes; protein abundance increases in response to DNA replication stress (2, 3, 4, 5, 6)
Name Description Regulatory Particle Non-ATPase 1
Chromosomal Location
ChrVI:153393 to 154313 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All RPN11 GO evidence and references
  View Computational GO annotations for RPN11
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 5 genes
Resources
Classical genetics
conditional
Large-scale survey
conditional
null
reduction of function
repressible
unspecified
Resources
1677 total interaction(s) for 876 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 1295
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 142
  • Biochemical Activity: 2
  • Co-crystal Structure: 2
  • Co-fractionation: 3
  • Co-purification: 9
  • PCA: 4
  • Reconstituted Complex: 37
  • Two-hybrid: 6

Genetic Interactions
  • Dosage Rescue: 3
  • Negative Genetic: 128
  • Phenotypic Enhancement: 1
  • Phenotypic Suppression: 1
  • Positive Genetic: 27
  • Synthetic Growth Defect: 2
  • Synthetic Haploinsufficiency: 1
  • Synthetic Lethality: 10
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 306
Molecular Weight (Da) 34,398
Isoelectric Point (pI) 6.13
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrVI:153393 to 154313 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..921 153393..154313 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001900
References cited on this page View Complete Literature Guide for RPN11
1) Finley D, et al.  (1998) Unified nomenclature for subunits of the Saccharomyces cerevisiae proteasome regulatory particle. Trends Biochem Sci 23(7):244-5
2) Verma R, et al.  (2002) Role of Rpn11 metalloprotease in deubiquitination and degradation by the 26S proteasome. Science 298(5593):611-5
3) Berndt C, et al.  (2002) Ubiquitin system: JAMMing in the name of the lid. Curr Biol 12(23):R815-7
4) Guterman A and Glickman MH  (2004) Complementary roles for Rpn11 and Ubp6 in deubiquitination and proteolysis by the proteasome. J Biol Chem 279(3):1729-38
5) Hofmann L, et al.  (2009) A nonproteolytic proteasome activity controls organelle fission in yeast. J Cell Sci 122(Pt 20):3673-83
6) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76