RPN11/YFR004W Summary

RPN11 BASIC INFORMATION

Standard Name	RPN11 1
Systematic Name	YFR004W
Alias	MPR1
Feature Type	ORF, Verified
Description	Metalloprotease subunit of the 19S regulatory particle of the 26S proteasome lid; couples the deubiquitination and degradation of proteasome substrates; involved, independent of catalytic activity, in fission of mitochondria and peroxisomes (2, 3, 4, 5)
Name Description	Regulatory Particle Non-ATPase 1

GO Annotations	All RPN11 GO evidence and references
	View Computational GO annotations for RPN11
Molecular Function
Manually curated	endopeptidase activity (TAS)
Biological Process
Manually curated	mitochondrial fission (IMP) peroxisome fission (IMP) ubiquitin-dependent protein catabolic process (TAS)
Cellular Component
Manually curated	cytosol (IDA) mitochondrion (IDA) nucleus (IDA) proteasome regulatory particle, lid subcomplex (IDA, IPI) proteasome storage granule (IDA)

Mutant Phenotype	All RPN11 Phenotype details and references
Classical genetics
conditional	heat sensitivity: increased mitochondrial morphology: abnormal peroxisomal distribution: abnormal
Large-scale survey
null	competitive fitness: decreased haploinsufficient inviable resistance to 2-phenyl-3-nitroso-imidazo[1,2-a]pyridine: decreased

Interactions	RPN11 All interactions details and references
	581 total interaction(s) for 420 unique genes/features.
Physical Interactions	Affinity Capture-MS: 477 Affinity Capture-RNA: 1 Affinity Capture-Western: 33 Co-fractionation: 2 Co-purification: 8 PCA: 3 Reconstituted Complex: 1 Two-hybrid: 6
Genetic Interactions	Dosage Rescue: 1 Phenotypic Enhancement: 2 Phenotypic Suppression: 1 Synthetic Growth Defect: 24 Synthetic Lethality: 1 Synthetic Rescue: 21

Sequence Information

ChrVI:153388 to 154308 | |

Last Update

Coordinates: 2003-09-26 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..921	153388..154308	2003-09-26	1996-07-31

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| UniProtKB

Primary SGDID	S000001900

RPN11 RESOURCES

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SGD ORF map	GBrowse

Click on histogram for expression summary
Expression Summary

ADDITIONAL INFORMATION for RPN11

NOMENCLATURE CONFLICT NOTE

Name	Relevance	Description
MPR1	Nomenclature conflict	Do not confuse RPN11/MPR1/YFR004W, a multicatalytic endopeptidase, with the MPR1 that encodes an N-acetyltransferase. Both have been referred to as MPR1. Note that the N-acetyltransferase is not present in the S288C strain.

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for RPN11]

1)	Finley D, et al. (1998) Unified nomenclature for subunits of the Saccharomyces cerevisiae proteasome regulatory particle. Trends Biochem Sci 23(7):244-5
2)	Verma R, et al. (2002) Role of Rpn11 metalloprotease in deubiquitination and degradation by the 26S proteasome. Science 298(5593):611-5
3)	Berndt C, et al. (2002) Ubiquitin system: JAMMing in the name of the lid. Curr Biol 12(23):R815-7
4)	Guterman A and Glickman MH (2004) Complementary roles for Rpn11 and Ubp6 in deubiquitination and proteolysis by the proteasome. J Biol Chem 279(3):1729-38
5)	Hofmann L, et al. (2009) A nonproteolytic proteasome activity controls organelle fission in yeast. J Cell Sci 122(Pt 20):3673-83

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