LOC1/YFR001W Summary Help

Standard Name LOC1 1
Systematic Name YFR001W
Feature Type ORF, Verified
Description Nuclear protein involved in asymmetric localization of ASH1 mRNA; binds double-stranded RNA in vitro; constituent of 66S pre-ribosomal particles; required at post-transcriptional step for efficient retrotransposition; absence results in decreased Ty1 Gag:GFP protein levels; relocalizes from nucleus to cytoplasm upon DNA replication stress (1, 2, 3, 4)
Name Description LOCalization of ASH1 mRNA 1
Chromosomal Location
ChrVI:149110 to 149724 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All LOC1 GO evidence and references
  View Computational GO annotations for LOC1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 3 genes
Resources
Classical genetics
null
Large-scale survey
null
overexpression
Resources
119 total interaction(s) for 87 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 83
  • Affinity Capture-RNA: 5
  • Affinity Capture-Western: 1
  • PCA: 9
  • Two-hybrid: 5

Genetic Interactions
  • Negative Genetic: 12
  • Phenotypic Enhancement: 1
  • Synthetic Growth Defect: 3

Resources
Expression Summary
histogram
Resources
Length (a.a.) 204
Molecular Weight (Da) 23,590
Isoelectric Point (pI) 11.1
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrVI:149110 to 149724 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..615 149110..149724 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001897
References cited on this page View Complete Literature Guide for LOC1
1) Long RM, et al.  (2001) An exclusively nuclear RNA-binding protein affects asymmetric localization of ASH1 mRNA and Ash1p in yeast. J Cell Biol 153(2):307-18
2) Horsey EW, et al.  (2004) Role of the yeast Rrp1 protein in the dynamics of pre-ribosome maturation. RNA 10(5):813-27
3) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
4) Risler JK, et al.  (2012) Host co-factors of the retrovirus-like transposon Ty1. Mob DNA 3(1):12