DEG1/YFL001W Summary Help

Standard Name DEG1 1
Systematic Name YFL001W
Alias PUS3 , HRM3 2
Feature Type ORF, Verified
Description tRNA:pseudouridine synthase; introduces pseudouridines at position 38 or 39 in tRNA, important for maintenance of translation efficiency and normal cell growth, localizes to both the nucleus and cytoplasm; non-essential for viability (1, 3, 4)
Name Description DEpressed Growth rate 1
Chromosomal Location
ChrVI:147131 to 148459 | ORF Map | GBrowse
Gbrowse
Genetic position: -1 cM
Gene Ontology Annotations All DEG1 GO evidence and references
  View Computational GO annotations for DEG1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 67 genes
Resources
Classical genetics
null
unspecified
Large-scale survey
null
overexpression
Resources
439 total interaction(s) for 300 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 1
  • Affinity Capture-RNA: 1
  • Biochemical Activity: 6
  • Reconstituted Complex: 1

Genetic Interactions
  • Dosage Rescue: 1
  • Negative Genetic: 300
  • Phenotypic Enhancement: 5
  • Phenotypic Suppression: 4
  • Positive Genetic: 104
  • Synthetic Growth Defect: 12
  • Synthetic Lethality: 3
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 442
Molecular Weight (Da) 50,888
Isoelectric Point (pI) 10.02
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrVI:147131 to 148459 | ORF Map | GBrowse
SGD ORF map
Genetic position: -1 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1329 147131..148459 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001895
References cited on this page View Complete Literature Guide for DEG1
1) Carbone ML, et al.  (1991) A gene tightly linked to CEN6 is important for growth of Saccharomyces cerevisiae. Curr Genet 19(1):1-8
2) Hepfer CE, et al.  (2005) DEG1, encoding the tRNA:pseudouridine synthase Pus3p, impacts HOT1-stimulated recombination in Saccharomyces cerevisiae. Mol Genet Genomics 274(5):528-38
3) Lecointe F, et al.  (1998) Characterization of yeast protein Deg1 as pseudouridine synthase (Pus3) catalyzing the formation of psi 38 and psi 39 in tRNA anticodon loop. J Biol Chem 273(3):1316-23
4) Lecointe F, et al.  (2002) Lack of pseudouridine 38/39 in the anticodon arm of yeast cytoplasmic tRNA decreases in vivo recoding efficiency. J Biol Chem 277(34):30445-53