SPB4/YFL002C Summary

SPB4 BASIC INFORMATION

Standard Name	SPB4
Systematic Name	YFL002C
Feature Type	ORF, Verified
Description	Putative ATP-dependent RNA helicase, nucleolar protein required for synthesis of 60S ribosomal subunits at a late step in the pathway; sediments with 66S pre-ribosomes in sucrose gradients (1)
Name Description	Suppressor of PAB1

GO Annotations	All SPB4 GO evidence and references
	View Computational GO annotations for SPB4
Molecular Function
Manually curated	ATP-dependent RNA helicase activity (TAS)
Biological Process
Manually curated	ribosomal large subunit assembly (TAS) rRNA processing (TAS)
Cellular Component
Manually curated	nucleolus (TAS)

Mutant Phenotype	All SPB4 Phenotype details and references
Classical genetics
dominant negative	7S pre-rRNA accumulation: increased vegetative growth: decreased
Large-scale survey
conditional	heat sensitivity: increased
null	inviable
repressible	mitochondrial morphology: abnormal

Interactions	SPB4 All interactions details and references
	84 total interaction(s) for 50 unique genes/features.
Physical Interactions	Affinity Capture-MS: 76 Affinity Capture-RNA: 1 Affinity Capture-Western: 2
Genetic Interactions	Phenotypic Enhancement: 2 Synthetic Growth Defect: 2 Synthetic Rescue: 1

Sequence Information

ChrVI:146929 to 145109 | |

Note: this feature is encoded on the Crick strand.

Last Update

Coordinates: 2003-09-26 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..1821	146929..145109	2003-09-26	1996-07-31

External Links	All Associated Seq \| E.C. \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| UniProtKB

Primary SGDID	S000001894

SPB4 RESOURCES

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SGD ORF map	GBrowse

Click on histogram for expression summary
Expression Summary

ADDITIONAL INFORMATION for SPB4

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for SPB4]

1)	de la Cruz J, et al. (1998) Spb4p, an essential putative RNA helicase, is required for a late step in the assembly of 60S ribosomal subunits in Saccharomyces cerevisiae. RNA 4(10):1268-81

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