SPB4/YFL002C Summary Help

Standard Name SPB4
Systematic Name YFL002C
Feature Type ORF, Verified
Description Putative ATP-dependent RNA helicase; nucleolar protein required for synthesis of 60S ribosomal subunits at a late step in the pathway; sediments with 66S pre-ribosomes in sucrose gradients (1, 2)
Name Description Suppressor of PAB1
Chromosomal Location
ChrVI:146934 to 145114 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All SPB4 GO evidence and references
  View Computational GO annotations for SPB4
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 3 genes
Resources
Classical genetics
dominant negative
null
Large-scale survey
conditional
null
reduction of function
repressible
Resources
452 total interaction(s) for 375 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 432
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 8

Genetic Interactions
  • Negative Genetic: 2
  • Positive Genetic: 1
  • Synthetic Growth Defect: 2
  • Synthetic Lethality: 4
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 606
Molecular Weight (Da) 69,422
Isoelectric Point (pI) 9.77
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrVI:146934 to 145114 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1821 146934..145114 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001894
References cited on this page View Complete Literature Guide for SPB4
1) de la Cruz J, et al.  (1998) Spb4p, an essential putative RNA helicase, is required for a late step in the assembly of 60S ribosomal subunits in Saccharomyces cerevisiae. RNA 4(10):1268-81
2) Garcia-Gomez JJ, et al.  (2011) Dynamics of the putative RNA helicase Spb4 during ribosome assembly in Saccharomyces cerevisiae. Mol Cell Biol 31(20):4156-64