BLM10/YFL007W Summary Help

Standard Name BLM10 1
Systematic Name YFL007W
Alias YFL006W
Feature Type ORF, Verified
Description Proteasome activator; binds the core proteasome (CP) and stimulates proteasome-mediated protein degradation by inducing gate opening; required for sequestering CP into proteasome storage granule (PSG) during quiescent phase and for nuclear import of CP in proliferating cells; required for resistance to bleomycin, may be involved in protecting against oxidative damage; similar to mammalian PA200 (1, 2, 3, 4, 5, 6, 7 and see Summary Paragraph)
Name Description BLeoMycin resistance 2, 8
Chromosomal Location
ChrVI:123479 to 129910 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All BLM10 GO evidence and references
  View Computational GO annotations for BLM10
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 7 genes
Resources
Classical genetics
null
Large-scale survey
null
Resources
145 total interaction(s) for 116 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 52
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 1
  • Co-fractionation: 1
  • Co-purification: 1
  • Two-hybrid: 1

Genetic Interactions
  • Negative Genetic: 67
  • Phenotypic Enhancement: 3
  • Phenotypic Suppression: 2
  • Positive Genetic: 8
  • Synthetic Growth Defect: 5
  • Synthetic Lethality: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 2,143
Molecular Weight (Da) 245,993
Isoelectric Point (pI) 5.77
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrVI:123479 to 129910 | ORF Map | GBrowse
This feature contains embedded feature(s): YFL006W | ARS604
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 2003-09-26
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..6432 123479..129910 2011-02-03 2003-09-26
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001887
SUMMARY PARAGRAPH for BLM10

BLM10 has been characterized as a proteasome activator protein that binds to the core proteasome particle to activate proteolysis (4) and as a factor which plays a role in the assembly of nuclear core proteasome particles (3). Fehlker et al. find Blm10p in association with proteasome precursor complexes (3). Schmidt et al. find that the majority of Blm10p in the cell is found in mature complexes containing both the core particle and the 19S regulatory module as well as Blm10p. They suggest that although most proteasome activators are multisubunit ring-shaped complexes, Blm10p is a large protein that appears to bind to the ring-shaped proteasome core particle as an individual protein (4).

Blm10p is similar to mammalian PA200 (1), a proteasome activator protein which stimulates the degradation of peptides, but not of intact proteins (5). PA200 is postulated to be involved in DNA repair, though the mechanism by which this may occur is not clear (5).

BLM3 was identified genetically in a large screen looking for mutations conferring hypersensitivity to the drug bleomycin and radiation (9, 8). The ORF YFL007W was cloned as a multicopy suppressor of the codominant blm3-1 mutation (2). Complete deletions of the YFL007W ORF indicated that cells are hypersensitive to bleomycin and viable in the absence of the drug (2). Subsequent resequencing of the region around YFL007W indicated the presence of an additional G, resulting in the original YFL006W ORF being merged into YFL007W (10, 11). Genetic mapping and sequencing of the expanded YFL007W ORF in the blm3-1 mutant indicated that YFL007W was an extragenic suppressor of the blm3-1 mutation (1). Thus, YFL007W was renamed BLM10 (1). The gene corresponding to the original blm3-1 mutation was later identified as UBP3 (12).

Last updated: 2006-03-13 Contact SGD

References cited on this page View Complete Literature Guide for BLM10
1) Doherty K, et al.  (2004) Expression of the expanded YFL007w ORF and assignment of the gene name BLM10. Yeast 21(12):1021-3
2) Febres DE, et al.  (2001) The novel BLM3 gene encodes a protein that protects against lethal effects of oxidative damage. Cell Mol Biol (Noisy-le-grand) 47(7):1149-62
3) Fehlker M, et al.  (2003) Blm3 is part of nascent proteasomes and is involved in a late stage of nuclear proteasome assembly. EMBO Rep 4(10):959-63
4) Schmidt M, et al.  (2005) The HEAT repeat protein Blm10 regulates the yeast proteasome by capping the core particle. Nat Struct Mol Biol 12(4):294-303
5) Ustrell V, et al.  (2002) PA200, a nuclear proteasome activator involved in DNA repair. EMBO J 21(13):3516-25
6) Dange T, et al.  (2011) Blm10 protein promotes proteasomal substrate turnover by an active gating mechanism. J Biol Chem 286(50):42830-9
7) Weberruss MH, et al.  (2013) Blm10 facilitates nuclear import of proteasome core particles. EMBO J 32(20):2697-707
8) Moore CW  (1991) Further characterizations of bleomycin-sensitive (blm) mutants of Saccharomyces cerevisiae with implications for a radiomimetic model. J Bacteriol 173(11):3605-8
9) Moore CW  (1980) Isolation and partial characterization of mutants of Saccharomyces cerevisiae altered in sensitivities to lethal effects of bleomycins. J Antibiot (Tokyo) 33(11):1369-75
10) Robben J, et al.  (2002) Revisiting the yeast chromosome VI DNA sequence reveals a correction merging YFL007w and YFL006w to a single ORF. Yeast 19(8):699-702
11) Kellis M, et al.  (2003) Sequencing and comparison of yeast species to identify genes and regulatory elements. Nature 423(6937):241-54
12) McCullock S, et al.  (2006) blm3-1 Is an Allele of UBP3, a Ubiquitin Protease that Appears to Act During Transcription of Damaged DNA. J Mol Biol 363(3):660-72