CDC4/YFL009W Summary Help

Standard Name CDC4 1
Systematic Name YFL009W
Feature Type ORF, Verified
Description F-box protein required for both the G1/S and G2/M phase transitions; modular substrate specificity factor which associates with core SCF (Cdc53p, Skp1p and Hrt1p/Rbx1p) to form the SCFCdc4 complex; SCFCdc4 acts as a ubiquitin-protein ligase directing ubiquitination of cyclin-dependent kinase (CDK) phosphorylated substrates, such as: Sic1p, Far1p, Cdc6p, Clb6p, and Cln3p (2, 3, 4, 5, 6, 7, 8, 9, 10)
Name Description Cell Division Cycle 11
Chromosomal Location
ChrVI:116145 to 118484 | ORF Map | GBrowse
Genetic position: -9 cM
Gene Ontology Annotations All CDC4 GO evidence and references
  View Computational GO annotations for CDC4
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 2 genes
Classical genetics
Large-scale survey
reduction of function
231 total interaction(s) for 124 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 18
  • Affinity Capture-Western: 37
  • Biochemical Activity: 14
  • Co-crystal Structure: 2
  • Co-fractionation: 1
  • Co-localization: 1
  • Co-purification: 3
  • Protein-peptide: 1
  • Reconstituted Complex: 26
  • Two-hybrid: 22

Genetic Interactions
  • Dosage Lethality: 5
  • Dosage Rescue: 4
  • Negative Genetic: 56
  • Phenotypic Enhancement: 1
  • Phenotypic Suppression: 9
  • Positive Genetic: 2
  • Synthetic Growth Defect: 8
  • Synthetic Lethality: 11
  • Synthetic Rescue: 10

Expression Summary
Length (a.a.) 779
Molecular Weight (Da) 86,089
Isoelectric Point (pI) 7.14
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrVI:116145 to 118484 | ORF Map | GBrowse
Genetic position: -9 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..2340 116145..118484 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001885
References cited on this page View Complete Literature Guide for CDC4
1) Hartwell LH  (1971) Genetic control of the cell division cycle in yeast. II. Genes controlling DNA replication and its initiation. J Mol Biol 59(1):183-94
2) Feldman RM, et al.  (1997) A complex of Cdc4p, Skp1p, and Cdc53p/cullin catalyzes ubiquitination of the phosphorylated CDK inhibitor Sic1p. Cell 91(2):221-30
3) Elsasser S, et al.  (1999) Phosphorylation controls timing of Cdc6p destruction: A biochemical analysis. Mol Biol Cell 10(10):3263-77
4) Goh PY and Surana U  (1999) Cdc4, a protein required for the onset of S phase, serves an essential function during G(2)/M transition in Saccharomyces cerevisiae. Mol Cell Biol 19(8):5512-22
5) Henchoz S, et al.  (1997) Phosphorylation- and ubiquitin-dependent degradation of the cyclin-dependent kinase inhibitor Far1p in budding yeast. Genes Dev 11(22):3046-60
6) Skowyra D, et al.  (1997) F-box proteins are receptors that recruit phosphorylated substrates to the SCF ubiquitin-ligase complex. Cell 91(2):209-19
7) Blondel M, et al.  (2000) Nuclear-specific degradation of Far1 is controlled by the localization of the F-box protein Cdc4. EMBO J 19(22):6085-97
8) Perkins G, et al.  (2001) Separate SCF(CDC4) recognition elements target Cdc6 for proteolysis in S phase and mitosis. EMBO J 20(17):4836-45
9) Jackson LP, et al.  (2006) Distinct mechanisms control the stability of the related S-phase cyclins Clb5 and Clb6. Mol Cell Biol 26(6):2456-66
10) Landry BD, et al.  (2012) F-box protein specificity for g1 cyclins is dictated by subcellular localization. PLoS Genet 8(7):e1002851
11) Hartwell LH, et al.  (1970) Genetic control of the cell-division cycle in yeast. I. Detection of mutants. Proc Natl Acad Sci U S A 66(2):352-9