HSP12/YFL014W Summary Help

Standard Name HSP12 1
Systematic Name YFL014W
Alias GLP1 , HOR5 2
Feature Type ORF, Verified
Description Plasma membrane protein involved in maintaining membrane organization; involved in maintaining organization during stress conditions; induced by heat shock, oxidative stress, osmostress, stationary phase, glucose depletion, oleate and alcohol; protein abundance increased in response to DNA replication stress and dietary restriction; regulated by the HOG and Ras-Pka pathways; required for dietary restriction-induced lifespan extension (1, 2, 3, 4, 5, 6, 7, 8, 9, 10)
Name Description Heat Shock Protein 1
Chromosomal Location
ChrVI:107256 to 107585 | ORF Map | GBrowse
Gene Ontology Annotations All HSP12 GO evidence and references
  View Computational GO annotations for HSP12
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 28 genes
Classical genetics
Large-scale survey
33 total interaction(s) for 29 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 10
  • Affinity Capture-RNA: 4
  • Biochemical Activity: 1
  • PCA: 1

Genetic Interactions
  • Negative Genetic: 14
  • Positive Genetic: 1
  • Synthetic Lethality: 1
  • Synthetic Rescue: 1

Expression Summary
Length (a.a.) 109
Molecular Weight (Da) 11,693
Isoelectric Point (pI) 5.1
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrVI:107256 to 107585 | ORF Map | GBrowse
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..330 107256..107585 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001880
References cited on this page View Complete Literature Guide for HSP12
1) Praekelt UM and Meacock PA  (1990) HSP12, a new small heat shock gene of Saccharomyces cerevisiae: analysis of structure, regulation and function. Mol Gen Genet 223(1):97-106
2) Hirayama T, et al.  (1995) Cloning and characterization of seven cDNAs for hyperosmolarity-responsive (HOR) genes of Saccharomyces cerevisiae. Mol Gen Genet 249(2):127-38
3) Stone RL, et al.  (1990) Cloning, sequencing and chromosomal assignment of a gene from Saccharomyces cerevisiae which is negatively regulated by glucose and positively by lipids. Gene 96(2):171-6
4) Siderius M, et al.  (1997) High-osmolarity signalling in Saccharomyces cerevisiae is modulated in a carbon-source-dependent fashion. Microbiology 143 ( Pt 10)():3241-50
5) Rep M, et al.  (1999) Osmotic stress-induced gene expression in Saccharomyces cerevisiae requires Msn1p and the novel nuclear factor Hot1p. Mol Cell Biol 19(8):5474-85
6) Garay-Arroyo A, et al.  (2000) Highly hydrophilic proteins in prokaryotes and eukaryotes are common during conditions of water deficit. J Biol Chem 275(8):5668-74
7) Sales K, et al.  (2000) The LEA-like protein HSP 12 in Saccharomyces cerevisiae has a plasma membrane location and protects membranes against desiccation and ethanol-induced stress. Biochim Biophys Acta 1463(2):267-78
8) Welker S, et al.  (2010) Hsp12 Is an Intrinsically Unstructured Stress Protein that Folds upon Membrane Association and Modulates Membrane Function. Mol Cell 39(4):507-520
9) Herbert AP, et al.  (2012) NMR structure of hsp12, a protein induced by and required for dietary restriction-induced lifespan extension in yeast. PLoS One 7(7):e41975
10) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76