HSP12/YFL014W Summary Help

Standard Name HSP12 1
Systematic Name YFL014W
Alias GLP1 , HOR5 2
Feature Type ORF, Verified
Description Plasma membrane protein involved in maintaining membrane organization; involved in maintaining organization during stress conditions; induced by heat shock, oxidative stress, osmostress, stationary phase, glucose depletion, oleate and alcohol; protein abundance increased in response to DNA replication stress and dietary restriction; regulated by the HOG and Ras-Pka pathways; required for dietary restriction-induced lifespan extension (1, 2, 3, 4, 5, 6, 7, 8, 9, 10)
Name Description Heat Shock Protein 1
Chromosomal Location
ChrVI:107256 to 107585 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All HSP12 GO evidence and references
  View Computational GO annotations for HSP12
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 28 genes
Resources
Classical genetics
null
overexpression
Large-scale survey
null
overexpression
Resources
33 total interaction(s) for 29 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 10
  • Affinity Capture-RNA: 4
  • Biochemical Activity: 1
  • PCA: 1

Genetic Interactions
  • Negative Genetic: 14
  • Positive Genetic: 1
  • Synthetic Lethality: 1
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 109
Molecular Weight (Da) 11,693
Isoelectric Point (pI) 5.1
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrVI:107256 to 107585 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..330 107256..107585 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001880
References cited on this page View Complete Literature Guide for HSP12
1) Praekelt UM and Meacock PA  (1990) HSP12, a new small heat shock gene of Saccharomyces cerevisiae: analysis of structure, regulation and function. Mol Gen Genet 223(1):97-106
2) Hirayama T, et al.  (1995) Cloning and characterization of seven cDNAs for hyperosmolarity-responsive (HOR) genes of Saccharomyces cerevisiae. Mol Gen Genet 249(2):127-38
3) Stone RL, et al.  (1990) Cloning, sequencing and chromosomal assignment of a gene from Saccharomyces cerevisiae which is negatively regulated by glucose and positively by lipids. Gene 96(2):171-6
4) Siderius M, et al.  (1997) High-osmolarity signalling in Saccharomyces cerevisiae is modulated in a carbon-source-dependent fashion. Microbiology 143 ( Pt 10)():3241-50
5) Rep M, et al.  (1999) Osmotic stress-induced gene expression in Saccharomyces cerevisiae requires Msn1p and the novel nuclear factor Hot1p. Mol Cell Biol 19(8):5474-85
6) Garay-Arroyo A, et al.  (2000) Highly hydrophilic proteins in prokaryotes and eukaryotes are common during conditions of water deficit. J Biol Chem 275(8):5668-74
7) Sales K, et al.  (2000) The LEA-like protein HSP 12 in Saccharomyces cerevisiae has a plasma membrane location and protects membranes against desiccation and ethanol-induced stress. Biochim Biophys Acta 1463(2):267-78
8) Welker S, et al.  (2010) Hsp12 Is an Intrinsically Unstructured Stress Protein that Folds upon Membrane Association and Modulates Membrane Function. Mol Cell 39(4):507-520
9) Herbert AP, et al.  (2012) NMR structure of hsp12, a protein induced by and required for dietary restriction-induced lifespan extension in yeast. PLoS One 7(7):e41975
10) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76