BUD27/YFL023W Summary

BUD27/YFL023W Summary

Standard Name	BUD27 1
Systematic Name	YFL023W
Alias	URI1 2
Feature Type	ORF, Verified
Description	Unconventional prefoldin protein involved in translation initiation; required for correct assembly of RNAP I, II, and III in an Rpb5p-dependent manner; shuttles between nucleus and cytoplasm; mutants have inappropriate expression of nutrient sensitive genes due to translational derepression of Gcn4p transcription factor; diploid mutants show random budding; ortholog of human URI/RMP (1, 3, 4, 5)
Name Description	BUD site selection 1

Chromosomal Location	ChrVI:90986 to 93376 \| ORF Map \| GBrowse

Gene Ontology Annotations All BUD27 GO evidence and references

	View Computational GO annotations for BUD27
Molecular Function
Manually curated	molecular_function unknown (ND)
Biological Process
Manually curated	formation of translation preinitiation complex (IGI, IMP)
Cellular Component
Manually curated	cytoplasm (IDA) colocalizes_with cytosolic ribosome (IDA)
High-throughput	cytoplasm (IDA)

Mutant phenotypes All BUD27 Phenotype evidence and references

Classical genetics
null	heat sensitivity: increased Rpa190p-HA distribution: abnormal Rpo31p-Myc distribution: abnormal Fus-Mid-GFP distribution: abnormal Rpb8p-ECFP distribution: abnormal Rpo21p distribution: abnormal resistance to cycloheximide: decreased resistance to paramomycin: decreased resistance to hygromycin B: decreased resistance to sirolimus: decreased resistance to tellurite: decreased vegetative growth: decreased rate viable
Large-scale survey
null	acid pH resistance: decreased bud morphology: abnormal budding pattern: abnormal cell shape: abnormal competitive fitness: decreased dessication resistance: decreased fermentative growth: decreased rate heat sensitivity: increased inviable mitotic recombination: increased Rad52-YFP distribution: increased Fus-Mid-GFP distribution: abnormal resistance to bleomycin: increased resistance to fenpropimorph: increased resistance to acetaldehyde: decreased resistance to ethanol: decreased resistance to propan-1-ol: decreased resistance to CTBT: decreased resistance to sodium arsenite: decreased resistance to dimethyl sulfoxide: decreased resistance to lovastatin: decreased resistance to camptothecin: increased resistance to caffeine: decreased resistance to cycloheximide: decreased resistance to hygromycin B: decreased resistance to sirolimus: decreased resistance to benzo[a]pyrene: decreased stress resistance: increased transposable element transposition: increased utilization of phosphorus source: decreased vacuolar morphology: abnormal vegetative growth: decreased rate
overexpression	resistance to sirolimus: decreased vegetative growth: decreased rate vegetative growth: decreased
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All BUD27 Interaction evidence and references

	325 total interaction(s) for 249 unique genes/features.
Physical Interactions	Affinity Capture-MS: 5 Affinity Capture-RNA: 1 Affinity Capture-Western: 5 Biochemical Activity: 12 PCA: 18 Reconstituted Complex: 9 Two-hybrid: 66
Genetic Interactions	Dosage Growth Defect: 2 Negative Genetic: 156 Phenotypic Enhancement: 2 Positive Genetic: 28 Synthetic Growth Defect: 13 Synthetic Lethality: 6 Synthetic Rescue: 2
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All BUD27 Protein evidence and references

Localization	YeastRC \| Organelle DB (UMich) \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| CGD Homologs \| CandidaDB Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrVI:90986 to 93376 | |

SGD ORF map

Last Update

Coordinates: 2011-02-03 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..2391	90986..93376	2011-02-03	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000001871

References cited on this page View Complete Literature Guide for BUD27

1)	Ni L and Snyder M (2001) A genomic study of the bipolar bud site selection pattern in Saccharomyces cerevisiae. Mol Biol Cell 12(7):2147-70
2)	Mockli N, et al. (2007) Yeast split-ubiquitin-based cytosolic screening system to detect interactions between transcriptionally active proteins. Biotechniques 42(6):725-30
3)	Gstaiger M, et al. (2003) Control of nutrient-sensitive transcription programs by the unconventional prefoldin URI. Science 302(5648):1208-12
4)	Deplazes A, et al. (2009) Yeast Uri1p promotes translation initiation and may provide a link to cotranslational quality control. EMBO J 28(10):1429-41
5)	Miron-Garcia MC, et al. (2013) The prefoldin bud27 mediates the assembly of the eukaryotic RNA polymerases in an rpb5-dependent manner. PLoS Genet 9(2):e1003297