RIM15/YFL033C Summary

RIM15/YFL033C Summary

Standard Name	RIM15 1
Systematic Name	YFL033C
Alias	TAK1
Feature Type	ORF, Verified
Description	Glucose-repressible protein kinase involved in signal transduction during cell proliferation in response to nutrients, specifically the establishment of stationary phase; identified as a regulator of IME2; substrate of Pho80p-Pho85p kinase (2, 3, 4, 5)
Name Description	Regulator of IME2 1

Chromosomal Location	ChrVI:74427 to 69115 \| ORF Map \| GBrowse
	Note: this feature is encoded on the Crick strand.

Gene Ontology Annotations All RIM15 GO evidence and references

	View Computational GO annotations for RIM15
Molecular Function
Manually curated	protein kinase activity (IDA) protein serine/threonine kinase activity (ISS)
High-throughput	protein kinase activity (IDA)
Biological Process
Manually curated	age-dependent response to oxidative stress involved in chronological cell aging (IMP) protein phosphorylation (IDA) regulation of meiosis (IMP) response to stress (IMP)
High-throughput	protein phosphorylation (IDA)
Cellular Component
Manually curated	cytoplasm (IDA) nucleus (IDA)
High-throughput	cytoplasm (IDA)

Regulatory Role

Regulatory modules	predicted: cellcycle (317)

Mutant phenotypes All RIM15 Phenotype evidence and references

Classical genetics
null	apoptosis: increased budding index: increased cell cycle progression in G1 phase: increased glycogen accumulation: decreased superoxide accumulation: increased chronological lifespan: decreased dessication resistance: absent dessication resistance: decreased entry into G0 (stationary phase): decreased heat sensitivity: increased hyperosmotic stress resistance: decreased metal resistance: decreased utilization of carbon source: decreased
repressible	chronological lifespan: decreased oxidative stress resistance: decreased
unspecified	sporulation efficiency: decreased
Large-scale survey
null	glycogen accumulation: decreased competitive fitness: increased dessication resistance: decreased resistance to sirolimus: decreased resistance to wortmannin: normal resistance to amitrole: decreased resistance to benzo[a]pyrene: increased resistance to cycloheximide: decreased resistance to benzo[a]pyrene: decreased sporulation: decreased sporulation efficiency: decreased vacuolar morphology: abnormal
overexpression	vegetative growth: decreased rate
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All RIM15 Interaction evidence and references

	396 total interaction(s) for 320 unique genes/features.
Physical Interactions	Affinity Capture-MS: 7 Affinity Capture-RNA: 2 Affinity Capture-Western: 4 Biochemical Activity: 20 Two-hybrid: 2
Genetic Interactions	Dosage Growth Defect: 1 Dosage Lethality: 1 Dosage Rescue: 4 Negative Genetic: 302 Phenotypic Enhancement: 4 Phenotypic Suppression: 10 Positive Genetic: 26 Synthetic Growth Defect: 1 Synthetic Lethality: 2 Synthetic Rescue: 10
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All RIM15 Protein evidence and references

Localization	YeastRC \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| AspGD Homologs \| CGD Homologs \| CandidaDB Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrVI:74427 to 69115 | |

Note: this feature is encoded on the Crick strand.

SGD ORF map

Last Update

Coordinates: 2011-02-03 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..5313	74427..69115	2011-02-03	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| E.C. \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000001861

References cited on this page View Complete Literature Guide for RIM15

1)	Su SS and Mitchell AP (1993) Identification of functionally related genes that stimulate early meiotic gene expression in yeast. Genetics 133(1):67-77
2)	Vidan S and Mitchell AP (1997) Stimulation of yeast meiotic gene expression by the glucose-repressible protein kinase Rim15p. Mol Cell Biol 17(5):2688-97
3)	Cameroni E, et al. (2004) The novel yeast PAS kinase Rim 15 orchestrates G0-associated antioxidant defense mechanisms. Cell Cycle 3(4):462-8
4)	Roosen J, et al. (2005) PKA and Sch9 control a molecular switch important for the proper adaptation to nutrient availability. Mol Microbiol 55(3):862-80
5)	Wanke V, et al. (2005) Regulation of G0 entry by the Pho80-Pho85 cyclin-CDK complex. EMBO J 24(24):4271-8