BAS1/YKR099W Summary Help

Standard Name BAS1 1
Systematic Name YKR099W
Feature Type ORF, Verified
Description Myb-related transcription factor involved in regulating basal and induced expression of genes of the purine and histidine biosynthesis pathways; also involved in regulation of meiotic recombination at specific genes (2, 3, 4 and see Summary Paragraph)
Name Description BASal 1
Chromosomal Location
ChrXI:635851 to 638286 | ORF Map | GBrowse
Gbrowse
Genetic position: 82 cM
Gene Ontology Annotations All BAS1 GO evidence and references
  View Computational GO annotations for BAS1
Molecular Function
Manually curated
High-throughput
Biological Process
Manually curated
Cellular Component
Manually curated
Regulatory Role
Binding motifs Predicted BAS1 Binding Site Locations
    Regulatory modules predicted: stressResponse (373)
    Resources
    Mutant phenotypes All BAS1 Phenotype evidence and references
    Classical genetics
    null
    Large-scale survey
    null
    overexpression
    Resources
    interactions All BAS1 Interaction evidence and references
    135 total interaction(s) for 117 unique genes/features.
    Physical Interactions
    • Affinity Capture-MS: 1
    • Affinity Capture-Western: 2
    • Protein-RNA: 1
    • Two-hybrid: 11
    Genetic Interactions
    • Dosage Growth Defect: 1
    • Negative Genetic: 71
    • Phenotypic Enhancement: 2
    • Phenotypic Suppression: 1
    • Positive Genetic: 39
    • Synthetic Growth Defect: 2
    • Synthetic Lethality: 2
    • Synthetic Rescue: 2
    Resources
    Expression Summary
    histogram
    Resources
    Protein Information All BAS1 Protein evidence and references
    Localization
    Phosphorylation PhosphoGRID | PhosphoPep Database
    Structure
    Homologs
    sequence information
    ChrXI:635851 to 638286 | ORF Map | GBrowse
    SGD ORF map
    Genetic position: 82 cM
    Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
    Subfeature details
    Relative
    Coordinates    		 Chromosomal
    Coordinates    		 Most Recent Updates
    Coordinates Sequence
    CDS 1..2436 635851..638286 2011-02-03 1996-07-31
    Retrieve sequences
    Analyze Sequence
    S288C only
    S288C vs. other species
    S288C vs. other strains
    Resources
    External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
    Primary SGDIDS000001807
    SUMMARY PARAGRAPH for BAS1

    BAS1 encodes a transcription factor involved in the expression of genes encoding enzymes acting in the histidine, purine, and pyrimidine biosynthetic pathways (5, 6, 1, 7, 1, 3). A genome-wide survey of genes regulated by Bas1p was performed by Denis et al. (5). Bas1p acts in conjunction with Grf10p (1) in a TATA-independent fashion (8). Bas1p has been shown to bind to a site containing a TGACTC hexanucleotide found in the ADE2 and ADE5,7 promoters (9). This motif is required for the regulation of ADE2 expression by Bas1p/Bas2p (3). A model for the action of Bas1p and Bas2p is that formation of a complex between the two proteins unmasks an activator function in Bas1p. Moreover, in adenine-replete cells, there may be a negative regulator that inhibits formation of this complex (10, 11). However, there is evidence that there may be other partners that can bind Bas1p (6). The N-terminal region of Bas1p is similar to those of proteins in the myb proto-oncogene family (2).

    Last updated: 1999-07-01

    References cited on this page View Complete Literature Guide for BAS1
    1) Arndt KT, et al.  (1987) Multiple global regulators control HIS4 transcription in yeast. Science 237(4817):874-80
    2) Tice-Baldwin K, et al.  (1989) BAS1 has a Myb motif and activates HIS4 transcription only in combination with BAS2. Science 246(4932):931-5
    3) Daignan-Fornier B and Fink GR  (1992) Coregulation of purine and histidine biosynthesis by the transcriptional activators BAS1 and BAS2. Proc Natl Acad Sci U S A 89(15):6746-50
    4) Mieczkowski PA, et al.  (2006) Global analysis of the relationship between the binding of the Bas1p transcription factor and meiosis-specific double-strand DNA breaks in Saccharomyces cerevisiae. Mol Cell Biol 26(3):1014-27
    5) Denis V, et al.  (1998) Role of the myb-like protein bas1p in Saccharomyces cerevisiae: a proteome analysis. Mol Microbiol 30(3):557-66
    6) Denis V and Daignan-Fornier B  (1998) Synthesis of glutamine, glycine and 10-formyl tetrahydrofolate is coregulated with purine biosynthesis in Saccharomyces cerevisiae. Mol Gen Genet 259(3):246-55
    7) Springer C, et al.  (1996) Amino acid and adenine cross-pathway regulation act through the same 5'-TGACTC-3' motif in the yeast HIS7 promoter. J Biol Chem 271(47):29637-43
    8) Pellman D, et al.  (1990) TATA-dependent and TATA-independent transcription at the HIS4 gene of yeast. Nature 348(6296):82-5
    9) Hovring I, et al.  (1994) DNA-binding domain and recognition sequence of the yeast BAS1 protein, a divergent member of the Myb family of transcription factors. J Biol Chem 269(26):17663-9
    10) Zhang F, et al.  (1997) Evidence that complex formation by Bas1p and Bas2p (Pho2p) unmasks the activation function of Bas1p in an adenine-repressible step of ADE gene transcription. Mol Cell Biol 17(6):3272-83
    11) Rolfes RJ, et al.  (1997) The transcriptional activators BAS1, BAS2, and ABF1 bind positive regulatory sites as the critical elements for adenine regulation of ADE5,7. J Biol Chem 272(20):13343-54
    12) Zhu C, et al.  (2009) High-resolution DNA-binding specificity analysis of yeast transcription factors. Genome Res 19(4):556-66
    13) Harbison CT, et al.  (2004) Transcriptional regulatory code of a eukaryotic genome. Nature 431(7004):99-104