MLP1/YKR095W Summary

MLP1/YKR095W Summary

Standard Name	MLP1 1 (see Nomenclature conflict Note)
Systematic Name	YKR095W
Alias	MPL1
Feature Type	ORF, Verified
Description	Myosin-like protein associated with the nuclear envelope; connects the nuclear pore complex with the nuclear interior; involved with Tel1p in telomere length control; involved with Pml1p and Pml39p in nuclear retention of unspliced mRNAs; MLP1 has a paralog, MLP2, that arose from the whole genome duplication (1, 2, 3, 4, 5, 6)
Name Description	Myosin-Like Protein 1

Chromosomal Location	ChrXI:619805 to 625432 \| ORF Map \| GBrowse

	Genetic position: 80.3 cM

Gene Ontology Annotations All MLP1 GO evidence and references

	View Computational GO annotations for MLP1
Molecular Function
Manually curated	ribonucleoprotein complex binding (IGI, IPI)
Biological Process
Manually curated	negative regulation of protein import into nucleus during spindle assembly checkpoint (IGI) nuclear retention of unspliced pre-mRNA at the site of transcription (IGI, IMP) poly(A)+ mRNA export from nucleus (IMP) protein import into nucleus (IGI) protein localization to nuclear pore (IMP) telomere tethering at nuclear periphery (IGI) transcriptional activation by promoter-terminator looping (IMP)
Cellular Component
Manually curated	nuclear envelope (IDA) nucleoplasm (IDA) colocalizes_with ribonucleoprotein complex (IPI) colocalizes_with spindle pole body (IDA)
High-throughput	integral to membrane (ISM)

Mutant phenotypes All MLP1 Phenotype evidence and references

Classical genetics
null	UV resistance: decreased nuclear export: abnormal Nab2p distribution: abnormal resistance to bleomycin A2: decreased telomere length: increased toxin resistance: increased
overexpression	poly(A) mRNA accumulation: increased
Large-scale survey
null	polyphosphate accumulation: abnormal competitive fitness: decreased haploinsufficient resistance to nickel sulfate: decreased resistance to doxorubicin: increased resistance to bleomycin: decreased resistance to cycloheximide: decreased resistance to caffeine: decreased resistance to fenpropimorph: decreased resistance to fluconazole: decreased resistance to tunicamycin: decreased resistance to methyl methanesulfonate: decreased resistance to benzo[a]pyrene: decreased vacuolar morphology: abnormal viable
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All MLP1 Interaction evidence and references

	158 total interaction(s) for 101 unique genes/features.
Physical Interactions	Affinity Capture-MS: 18 Affinity Capture-RNA: 3 Affinity Capture-Western: 9 Biochemical Activity: 1 Co-localization: 1 PCA: 2 Reconstituted Complex: 4 Two-hybrid: 4
Genetic Interactions	Dosage Growth Defect: 1 Dosage Rescue: 1 Negative Genetic: 69 Phenotypic Enhancement: 7 Phenotypic Suppression: 4 Positive Genetic: 13 Synthetic Growth Defect: 5 Synthetic Haploinsufficiency: 1 Synthetic Lethality: 7 Synthetic Rescue: 8
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All MLP1 Protein evidence and references

Localization	YeastRC \| Organelle DB (UMich) \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| AspGD Homologs \| CGD Homologs \| CandidaDB Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrXI:619805 to 625432 | |

SGD ORF map

: 80.3 cM

Last Update

Coordinates: 2011-02-03 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..5628	619805..625432	2011-02-03	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000001803

NOMENCLATURE CONFLICT NOTE

Name	Relevance	Description
KDX1	Nomenclature conflict	Both MLP1/YKR095W and YKL161C have been referred to as MLP1.

References cited on this page View Complete Literature Guide for MLP1

1)	Kolling R, et al. (1993) A new yeast gene with a myosin-like heptad repeat structure. Mol Gen Genet 237(3):359-69
2)	Strambio-de-Castillia C, et al. (1999) Proteins connecting the nuclear pore complex with the nuclear interior. J Cell Biol 144(5):839-55
3)	Hediger F, et al. (2002) Myosin-like proteins 1 and 2 are not required for silencing or telomere anchoring, but act in the Tel1 pathway of telomere length control. J Struct Biol 140(1-3):79-91
4)	Galy V, et al. (2004) Nuclear retention of unspliced mRNAs in yeast is mediated by perinuclear Mlp1. Cell 116(1):63-73
5)	Palancade B, et al. (2005) Pml39, a novel protein of the nuclear periphery required for nuclear retention of improper messenger ribonucleoparticles. Mol Biol Cell 16(11):5258-68
6)	Byrne KP and Wolfe KH (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61